3D Printed Wavy Scaffolds Enhance Mesenchymal Stem Cell Osteogenesis.
additive manufacturing
biomaterials
bone regeneration
stem cells
tissue engineering
Journal
Micromachines
ISSN: 2072-666X
Titre abrégé: Micromachines (Basel)
Pays: Switzerland
ID NLM: 101640903
Informations de publication
Date de publication:
25 Dec 2019
25 Dec 2019
Historique:
received:
22
11
2019
revised:
19
12
2019
accepted:
21
12
2019
entrez:
29
12
2019
pubmed:
29
12
2019
medline:
29
12
2019
Statut:
epublish
Résumé
There is a growing interest in developing 3D porous scaffolds with tunable architectures for bone tissue engineering. Surface topography has been shown to control stem cell behavior including differentiation. In this study, we printed 3D porous scaffolds with wavy or linear patterns to investigate the effect of wavy scaffold architecture on human mesenchymal stem cell (hMSC) osteogenesis. Five distinct wavy scaffolds were designed using sinusoidal waveforms with varying wavelengths and amplitudes, and orthogonal scaffolds were designed using linear patterns. We found that hMSCs attached to wavy patterns, spread by taking the shape of the curvatures presented by the wavy patterns, exhibited an elongated shape and mature focal adhesion points, and differentiated into the osteogenic lineage. When compared to orthogonal scaffolds, hMSCs on wavy scaffolds showed significantly enhanced osteogenesis, indicated by higher calcium deposition, alkaline phosphatase activity, and osteocalcin staining. This study aids in the development of 3D scaffolds with novel architectures to direct stem osteogenesis for bone tissue engineering.
Identifiants
pubmed: 31881771
pii: mi11010031
doi: 10.3390/mi11010031
pmc: PMC7019315
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : National Science Foundation
ID : DMR: 1714882; CMMI: 15-48571
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Acta Biomater. 2013 Apr;9(4):5989-96
pubmed: 23232210
J Biomed Mater Res A. 2014 Oct;102(10):3379-92
pubmed: 24132871
Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2130-5
pubmed: 19179282
Tissue Eng Part A. 2009 Feb;15(2):205-19
pubmed: 18694293
Biomaterials. 2019 Jan;190-191:97-110
pubmed: 30415019
J Mater Sci Mater Med. 2005 Sep;16(9):807-19
pubmed: 16167109
Nat Mater. 2007 Dec;6(12):997-1003
pubmed: 17891143
Sci Transl Med. 2015 Apr 29;7(285):285ra64
pubmed: 25925683
Biomaterials. 2007 May;28(15):2491-504
pubmed: 17335896
J Orthop Surg Res. 2014 Mar 17;9(1):18
pubmed: 24628910
Biofabrication. 2011 Sep;3(3):034103
pubmed: 21725148
Biomaterials. 2006 Jun;27(16):3115-24
pubmed: 16458961
Science. 2005 Nov 18;310(5751):1139-43
pubmed: 16293750
Biomed Mater. 2010 Dec;5(6):062001
pubmed: 20924139
Biomaterials. 1999 Mar;20(6):573-88
pubmed: 10213360
Injury. 2011 Sep;42 Suppl 2:S77-81
pubmed: 21724186
Med Eng Phys. 2014 Apr;36(4):448-57
pubmed: 24636449
J Biomed Mater Res B Appl Biomater. 2014 Aug;102(6):1304-15
pubmed: 24425207
J Tissue Eng Regen Med. 2012 Feb;6(2):155-62
pubmed: 21446054
Dev Cell. 2004 Apr;6(4):483-95
pubmed: 15068789
Stem Cell Res Ther. 2010 May 04;1(2):10
pubmed: 20637059
Acta Biomater. 2016 Dec;46:3-14
pubmed: 27667018
Biomaterials. 2005 Sep;26(27):5474-91
pubmed: 15860204
Nat Biotechnol. 2016 Mar;34(3):312-9
pubmed: 26878319
Stem Cells. 2008 Nov;26(11):2921-7
pubmed: 18703661
PLoS One. 2012;7(5):e36336
pubmed: 22606256
Acta Biomater. 2010 Aug;6(8):3349-59
pubmed: 20132912
J Mater Sci Mater Med. 2009 Jan;20(1):271-9
pubmed: 18763023
J Biomed Mater Res. 2001 May;55(2):203-16
pubmed: 11255172
Biomaterials. 2010 Sep;31(25):6511-8
pubmed: 20541257
Biofabrication. 2017 Jun 07;9(2):024102
pubmed: 28589921
Adv Mater. 2010 Jan 12;22(2):175-89
pubmed: 20217683
Nat Commun. 2012 Apr 24;3:792
pubmed: 22531177
Biomed Mater. 2019 Sep 09;14(6):065003
pubmed: 31382255
Dent Mater. 2012 Feb;28(2):113-22
pubmed: 22047943
Biomaterials. 2003 May;24(12):2077-82
pubmed: 12628828
J Biomed Mater Res. 2002 Mar 15;59(4):676-81
pubmed: 11774329
Biomaterials. 2015 Jun;52:140-7
pubmed: 25818420
J Biomed Mater Res A. 2007 Nov;83(2):434-45
pubmed: 17465019
Sci Rep. 2018 Apr 30;8(1):6744
pubmed: 29712979
Cell. 2006 Aug 25;126(4):677-89
pubmed: 16923388
Biomater Sci. 2019 Jan 29;7(2):560-570
pubmed: 30534726
Acta Biomater. 2015 Dec;28:64-75
pubmed: 26432440
Science. 1999 Apr 2;284(5411):143-7
pubmed: 10102814
J Biomed Mater Res. 1999 Oct;47(1):8-17
pubmed: 10400875
Trends Biotechnol. 2004 Jul;22(7):354-62
pubmed: 15245908
Acta Biomater. 2019 May;90:37-48
pubmed: 30905862
Biomaterials. 2005 Aug;26(23):4817-27
pubmed: 15763261
Acta Biomater. 2014 Feb;10(2):580-94
pubmed: 24184176
J Biomed Mater Res. 1998 Dec 15;42(4):491-9
pubmed: 9827671
Curr Opin Biotechnol. 2013 Oct;24(5):841-6
pubmed: 23545441
J Biomed Mater Res B Appl Biomater. 2013 May;101(4):610-9
pubmed: 23281260
Bone. 2010 Feb;46(2):386-95
pubmed: 19800045
Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11594-9
pubmed: 16049098
J Cell Biol. 2007 Dec 3;179(5):1043-57
pubmed: 18056416
Trends Biotechnol. 2012 Oct;30(10):546-54
pubmed: 22939815
Biomech Model Mechanobiol. 2018 Oct;17(5):1481-1495
pubmed: 29876780
Sci Transl Med. 2016 Sep 28;8(358):358ra127
pubmed: 27683552
J Mater Sci Mater Med. 2008 Jul;19(7):2555-61
pubmed: 17665100
Eur Spine J. 2001 Oct;10 Suppl 2:S96-101
pubmed: 11716023
ACS Biomater Sci Eng. 2016 Oct 10;2(10):1679-1693
pubmed: 28025653
Adv Healthc Mater. 2013 Jan;2(1):155-64
pubmed: 23184470
J R Soc Interface. 2010 Feb 6;7(43):209-27
pubmed: 19864265
Virtual Phys Prototyp. 2012 Mar 1;7(1):13-24
pubmed: 23066427
Tissue Eng Part A. 2012 Apr;18(7-8):816-27
pubmed: 22011243
Nano Today. 2014 Dec 1;9(6):759-784
pubmed: 25883674
J R Soc Interface. 2008 Oct 6;5(27):1173-80
pubmed: 18348957
Acta Biomater. 2007 Nov;3(6):1007-18
pubmed: 17627910
Biomaterials. 2011 Dec;32(35):9188-96
pubmed: 21890197
Biomaterials. 2010 Jan;31(3):461-6
pubmed: 19819008
Stem Cells Int. 2016;2016:6180487
pubmed: 26880976
Science. 2009 Jun 26;324(5935):1673-7
pubmed: 19556500
Craniomaxillofac Trauma Reconstr. 2009 Oct;2(3):125-34
pubmed: 22110806
Proc Inst Mech Eng H. 2010 Dec;224(12):1441-53
pubmed: 21287830
Biomaterials. 2016 Mar;83:363-82
pubmed: 26803405
Acta Biomater. 2009 Oct;5(8):2884-93
pubmed: 19447211
Indian J Med Res. 2010 Jul;132:15-30
pubmed: 20693585
Proc Natl Acad Sci U S A. 2010 Mar 16;107(11):4872-7
pubmed: 20194780
Sci Rep. 2019 Jun 24;9(1):9170
pubmed: 31235704
Mater Sci Eng C Mater Biol Appl. 2019 Mar;96:153-165
pubmed: 30606521
Nat Mater. 2005 Jul;4(7):518-24
pubmed: 16003400
MRS Commun. 2017 Sep;7(3):383-390
pubmed: 31192033