Crebl2 regulates cell metabolism in muscle and liver cells.
Activating Transcription Factor 6
/ genetics
Animals
Cell Line
Cell Proliferation
/ physiology
Cyclic AMP Response Element-Binding Protein
/ metabolism
Female
Hepatocytes
/ metabolism
Humans
Liver
/ metabolism
Male
Mechanistic Target of Rapamycin Complex 1
/ metabolism
Mice
Mice, Knockout
Muscles
/ metabolism
Myoblasts
/ metabolism
Signal Transduction
/ genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
27 12 2019
27 12 2019
Historique:
received:
19
07
2019
accepted:
11
12
2019
entrez:
29
12
2019
pubmed:
29
12
2019
medline:
11
11
2020
Statut:
epublish
Résumé
We previously identified Drosophila REPTOR and REPTOR-BP as transcription factors downstream of mTORC1 that play an important role in regulating organismal metabolism. We study here the mammalian ortholog of REPTOR-BP, Crebl2. We find that Crebl2 mediates part of the transcriptional induction caused by mTORC1 inhibition. In C2C12 myoblasts, Crebl2 knockdown leads to elevated glucose uptake, elevated glycolysis as observed by lactate secretion, and elevated triglyceride biosynthesis. In Hepa1-6 hepatoma cells, Crebl2 knockdown also leads to elevated triglyceride levels. In sum, this works identifies Crebl2 as a regulator of cellular metabolism that can link nutrient sensing via mTORC1 to the metabolic response of cells.
Identifiants
pubmed: 31882710
doi: 10.1038/s41598-019-56407-w
pii: 10.1038/s41598-019-56407-w
pmc: PMC6934747
doi:
Substances chimiques
Activating Transcription Factor 6
0
Atf6b protein, mouse
0
Cyclic AMP Response Element-Binding Protein
0
Mechanistic Target of Rapamycin Complex 1
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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