Catalytic hairpin assembly-based double-end DNAzyme cascade-feedback amplification for sensitive fluorescence detection of HIV-1 DNA.

Biosensing Techniques / methods DNA, Catalytic / chemistry DNA, Viral / analysis Fluorescent Dyes / chemistry HIV Infections / blood HIV-1 / isolation & purification Humans Nucleic Acid Amplification Techniques / methods Spectrometry, Fluorescence / methods
Catalytic hairpin assembly DNAzyme Fluorescence detection HIV-1 DNA Signal amplification

Journal

Analytica chimica acta
ISSN: 1873-4324
Titre abrégé: Anal Chim Acta
Pays: Netherlands
ID NLM: 0370534

Informations de publication

Date de publication:
01 Feb 2020
Historique:
received: 06 09 2019
revised: 18 10 2019
accepted: 19 10 2019
entrez: 30 12 2019
pubmed: 31 12 2019
medline: 4 6 2020
Statut: ppublish

Résumé

In this work, a simple all-nucleic acid cascade-feedback amplification strategy for homogeneous and protein enzyme-free fluorescence detection of HIV-1 related DNA (HIV-1 DNA) has been proposed by integrating catalytic hairpin assembly (CHA) circuit with double-end Mg

Identifiants

DOI: 10.1016/j.aca.2019.10.051 PMID: 31883582
pubmed: 31883582
pii: S0003-2670(19)31278-4
doi: 10.1016/j.aca.2019.10.051
pii:
doi:

Substances chimiques

DNA, Catalytic 0
DNA, Viral 0
Fluorescent Dyes 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

159-165

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Xiaoyu Liu (X)

Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, School of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China.

Xiaomei Zhou (X)

Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, School of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China.

Xinyu Xia (X)

Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, School of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China.

Hua Xiang (H)

Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, School of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address: xianghua@cqmu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › Catalytic hairpin assembly-based double-end...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN catalytique › Catalytic hairpin assembly-based double-end...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN viral › Catalytic hairpin assembly-based double-end...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Catalytic hairpin assembly-based double-end...
questionsmedicales.fr › Maladies du système immunitaire › Déficits immunitaires › Infections à VIH › Catalytic hairpin assembly-based double-end...
questionsmedicales.fr › Virus › Virus à ARN › Retroviridae › Lentivirus › Lentivirus des primates › VIH (Virus de l'Immunodéficience Humaine) › VIH-1 (Virus de l'Immunodéficience Humaine de type 1) › Catalytic hairpin assembly-based double-end...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Catalytic hairpin assembly-based double-end...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Catalytic hairpin assembly-based double-end...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectrométrie de fluorescence › Catalytic hairpin assembly-based double-end...