The Transmembrane Protein Semi1 Positions Gamete Nuclei for Reciprocal Fertilization in Tetrahymena.
Developmental Genetics
Genetics
Molecular Biology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
24 Jan 2020
24 Jan 2020
Historique:
received:
10
09
2019
revised:
01
11
2019
accepted:
25
11
2019
pubmed:
31
12
2019
medline:
31
12
2019
entrez:
30
12
2019
Statut:
ppublish
Résumé
During sexual reproduction in the ciliate, Tetrahymena thermophila, cells of complementary mating type pair ("conjugate") undergo simultaneous meiosis and fertilize each other. In both mating partners only one of the four meiotic products is "selected" to escape autophagy, and this nucleus divides mitotically to produce two pronuclei. The migrating pronucleus of one cell translocates to the mating partner and fuses with its stationary pronucleus and vice versa. Selection of the designated gametic nucleus was thought to depend on its position within the cell because it always attaches to the junction with the partner cell. Here we show that a transmembrane protein, Semi1, is crucial for attachment. Loss of Semi1 causes failure to attach and consequent infertility. However, a nucleus is selected and gives rise to pronuclei regardless of Semi1 expression, indicating that attachment of a nucleus to the junction is not a precondition for selection but follows the selection process.
Identifiants
pubmed: 31884169
pii: S2589-0042(19)30494-8
doi: 10.1016/j.isci.2019.100749
pmc: PMC6941865
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100749Subventions
Organisme : Austrian Science Fund FWF
ID : P 27313
Pays : Austria
Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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