Live Imaging of Organelle Motility in RPE Flatmounts.

Humans Lysosomes / physiology Microscopy, Confocal Phagocytosis Phagosomes / physiology Retinal Photoreceptor Cell Outer Segment / physiology Retinal Pigment Epithelium / cytology
Flatmount Live imaging LysoTracker Red Lysosome Phagosome RHO-EGFP mice RPE Spinning disk confocal microscopy

Journal

Advances in experimental medicine and biology
ISSN: 0065-2598
Titre abrégé: Adv Exp Med Biol
Pays: United States
ID NLM: 0121103

Informations de publication

Date de publication:
2019
Historique:
entrez: 30 12 2019
pubmed: 31 12 2019
medline: 6 2 2020
Statut: ppublish

Résumé

The retinal pigment epithelium (RPE) performs several functions that are crucial for normal retinal function and vision, including the daily phagocytosis of photoreceptor outer segment (POS) membranes. Defects in the motility and degradation of POS phagosomes may be associated with some inherited and age-related retinal degenerations. Given the apical to basal translocation of phagosomes during maturation and degradation, studies of the underlying mechanisms require analyses of the dynamics in 3-D. In this chapter, we report a method for investigating the 3-D motility of POS phagosomes and lysosomes, utilizing high-speed, spinning disk confocal microscopy of live RPE flatmounts.

Identifiants

DOI: 10.1007/978-3-030-27378-1_64 PMID: 31884643
pubmed: 31884643
doi: 10.1007/978-3-030-27378-1_64
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

389-393

Auteurs

Ankita Umapathy (A)

Department of Ophthalmology and Stein Eye Institute, University of California, Los Angeles (UCLA),, Los Angeles, CA, USA.

David S Williams (DS)

Department of Ophthalmology and Stein Eye Institute, University of California, Los Angeles (UCLA), Los Angeles, CA, USA. dswilliams@ucla.edu.
Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles (UCLA), Los Angeles, CA, USA. dswilliams@ucla.edu.
Molecular Biology Institute, David Geffen School of Medicine, University of California, Los Angeles (UCLA), Los Angeles, CA, USA. dswilliams@ucla.edu.
Brain Research Institute, David Geffen School of Medicine, University of California, Los Angeles (UCLA), Los Angeles, CA, USA. dswilliams@ucla.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Live Imaging of Organelle Motility in RPE Flatmounts.
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Vésicules cytoplasmiques › Lysosomes › Live Imaging of Organelle Motility in RPE Flatmounts.
questionsmedicales.fr › Techniques d'investigation › Microscopie confocale › Live Imaging of Organelle Motility in RPE Flatmounts.
questionsmedicales.fr › Phénomènes du système immunitaire › Phagocytose › Live Imaging of Organelle Motility in RPE Flatmounts.
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Vésicules cytoplasmiques › Phagosomes › Live Imaging of Organelle Motility in RPE Flatmounts.
questionsmedicales.fr › Cellules › Neurones › Neurones afférents › Cellules réceptrices sensorielles › Cellules photoréceptrices › Cellules photoréceptrices de vertébré › Segment externe de cellule photoréceptrice rétinienne › Live Imaging of Organelle Motility in RPE Flatmounts.
questionsmedicales.fr › Organes des sens › Oeil › Rétine › Épithélium pigmentaire de la rétine › Live Imaging of Organelle Motility in RPE Flatmounts.