Analysis of Damage and Wound Healing in the Retinal Pigmented Epithelium.
Best1
Confetti mouse line
Cre
Flat-mounting
Histopathology
Inherited retinal diseases
Noninvasive imaging
Retinal pigmented epithelium (RPE)
Transgenic mice
Journal
Advances in experimental medicine and biology
ISSN: 0065-2598
Titre abrégé: Adv Exp Med Biol
Pays: United States
ID NLM: 0121103
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
30
12
2019
pubmed:
31
12
2019
medline:
6
2
2020
Statut:
ppublish
Résumé
Previous studies of retinal pigment epithelium (RPE) morphology found cell-level and spatial patterning differences in many quantitative metrics in comparing normal and disease conditions. However, most of these studies examined eyes from deceased animals. Here we sought to compare noninvasively imaged RPE cells from live mice to histopathology. We describe changes to improve noninvasive imaging of RPE in the live mouse. In retinal diseases, there can be invasion by Iba1-positive cells, which can be detected by noninvasive imaging techniques. Here we can detect potential Iba1-positive cells at the level of the RPE noninvasively.
Identifiants
pubmed: 31884649
doi: 10.1007/978-3-030-27378-1_70
pmc: PMC7362297
mid: NIHMS1603414
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
425-430Subventions
Organisme : NEI NIH HHS
ID : R01 EY028859
Pays : United States
Organisme : NEI NIH HHS
ID : P30 EY006360
Pays : United States
Organisme : RRD VA
ID : I21 RX001924
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC009246
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY021592
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY028450
Pays : United States
Organisme : NEI NIH HHS
ID : F31 EY028855
Pays : United States
Organisme : NEI NIH HHS
ID : T32 EY007092
Pays : United States
Organisme : RRD VA
ID : I01 RX002806
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008490
Pays : United States
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