High resolution melting based method for rapid discriminatory diagnosis of co-infecting Leptomonas seymouri in Leishmania donovani-induced leishmaniasis.
Diagnosis
HSP70 gene
High resolution melting
Leishmania donovani
Leptomonas seymouri
Melting peak
Visceral leishmaniasis
Journal
Parasitology international
ISSN: 1873-0329
Titre abrégé: Parasitol Int
Pays: Netherlands
ID NLM: 9708549
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
13
05
2019
revised:
02
09
2019
accepted:
26
12
2019
pubmed:
31
12
2019
medline:
22
8
2020
entrez:
31
12
2019
Statut:
ppublish
Résumé
Leishmania donovani, a protozoan parasite of family Trypanosomatidae, causes fatal visceral leishmaniasis (VL) in the Indian subcontinent and Africa and cutaneous leishmaniasis (CL) in Sri Lanka. Another member of Trypanosomatidae, Leptomonas seymouri, resembling Leishmania was discovered recently to co-exist with L. donovani in the clinical samples from India and Sri Lanka and therefore, interfere with its investigations. We earlier described a method for selective elimination of such co-existing L. seymouri from clinical samples of VL exploiting the differential growth of the parasites at 37 °C in vitro. Here, we explored ways for a rapid discriminatory diagnosis using high resolution melting (HRM) curves to detect co-occurring L. seymouri with L. donovani in clinical samples. Initial attempt with kDNA-minicircle (mitochondrial DNA) based HRM did not display different T
Identifiants
pubmed: 31887394
pii: S1383-5769(19)30398-8
doi: 10.1016/j.parint.2019.102047
pmc: PMC7189336
mid: NIHMS1576837
pii:
doi:
Substances chimiques
DNA, Kinetoplast
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102047Subventions
Organisme : NIAID NIH HHS
ID : R01 AI099602
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI136033
Pays : United States
Informations de copyright
Copyright © 2019. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no conflicts of interest with the contents of this article.
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