Data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag.

Activity ratio Enzyme tag Fusion expression HTP screening Mutant libraries

Journal

Data in brief
ISSN: 2352-3409
Titre abrégé: Data Brief
Pays: Netherlands
ID NLM: 101654995

Informations de publication

Date de publication:
Feb 2020
Historique:
received: 27 10 2019
revised: 01 12 2019
accepted: 04 12 2019
entrez: 1 1 2020
pubmed: 1 1 2020
medline: 1 1 2020
Statut: epublish

Résumé

Data in this article are associated with the research article "High-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag" (Li et al., 2019) [1]. Data are provided on the development of a system for high-throughput (HTP) screening of mutants through the comparison of the activity ratios of an applicable enzyme and its mutants to a suitable tag enzyme in cell lysates of their fused forms, with

Identifiants

pubmed: 31890819
doi: 10.1016/j.dib.2019.104985
pii: S2352-3409(19)31340-X
pii: 104985
pmc: PMC6931123
doi:

Types de publication

Journal Article

Langues

eng

Pagination

104985

Informations de copyright

© 2019 The Authors.

Références

Anal Chem. 2013 Feb 19;85(4):2143-54
pubmed: 23305208
Anal Sci. 2015;31(5):421-7
pubmed: 25958872
Anal Biochem. 2020 Jan 1;588:113474
pubmed: 31614116

Auteurs

Yaping Li (Y)

School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.

Huimin Chong (H)

School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.

Xiang Zhang (X)

School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.

Xiaolan Yang (X)

School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.

Fei Liao (F)

School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, China.

Classifications MeSH