Metformin Inhibits Proliferation and Tumor Growth of QGP-1 Pancreatic Neuroendocrine Tumor Cells by Inducing Cell Cycle Arrest and Apoptosis.


Journal

Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988

Informations de publication

Date de publication:
Jan 2020
Historique:
received: 08 11 2019
revised: 22 11 2019
accepted: 25 11 2019
entrez: 2 1 2020
pubmed: 2 1 2020
medline: 15 1 2020
Statut: ppublish

Résumé

Pancreatic neuroendocrine tumors (pNETs) are rare pancreatic neoplasms, and therapeutic options for pNETs are limited. Metformin is an anti-hypoglycemic drug that appears to have anticancer effects. However, little is known about the effect of metformin on pNETs. In this study, we investigated the anti-proliferative effect of metformin on a human pNET cell line. The anti-proliferative properties of metformin were evaluated in QGP-1 and NCI-H727 cells using a cell counting kit-8 assay. Xenograft mouse models were used to assess the tumor effect in vivo. Metformin inhibited the proliferation and anti-tumor growth of QGP-1 cells, accompanied by their arrest during the cell cycle at the G Taken together, our study demonstrated the therapeutic potential of metformin and provides molecular mechanistic insights into its anti-tumoral effect on pNETs. This study is the first one describing anti-tumoral effects in pNETs.

Sections du résumé

BACKGROUND/AIM OBJECTIVE
Pancreatic neuroendocrine tumors (pNETs) are rare pancreatic neoplasms, and therapeutic options for pNETs are limited. Metformin is an anti-hypoglycemic drug that appears to have anticancer effects. However, little is known about the effect of metformin on pNETs. In this study, we investigated the anti-proliferative effect of metformin on a human pNET cell line.
MATERIALS AND METHODS METHODS
The anti-proliferative properties of metformin were evaluated in QGP-1 and NCI-H727 cells using a cell counting kit-8 assay. Xenograft mouse models were used to assess the tumor effect in vivo.
RESULTS RESULTS
Metformin inhibited the proliferation and anti-tumor growth of QGP-1 cells, accompanied by their arrest during the cell cycle at the G
CONCLUSION CONCLUSIONS
Taken together, our study demonstrated the therapeutic potential of metformin and provides molecular mechanistic insights into its anti-tumoral effect on pNETs. This study is the first one describing anti-tumoral effects in pNETs.

Identifiants

pubmed: 31892560
pii: 40/1/121
doi: 10.21873/anticanres.13933
doi:

Substances chimiques

Biomarkers 0
MicroRNAs 0
Metformin 9100L32L2N

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

121-132

Informations de copyright

Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Auteurs

Hiroki Yamana (H)

Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.

Kiyohito Kato (K)

Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.

Hideki Kobara (H)

Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.

Shintaro Fujihara (S)

Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.

Koji Fujita (K)

Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.

Daisuke Namima (D)

Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.

Naoki Fujita (N)

Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.

Kiyoyuki Kobayashi (K)

Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.

Hideki Kamada (H)

Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.

Asahiro Morishita (A)

Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.

Kunihiko Tsutsui (K)

Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.

Hisakazu Iwama (H)

Life Science Research Center, Kagawa University, Kagawa, Japan.

Tsutomu Masaki (T)

Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan tmasaki@med.kagawa-u.ac.jp.

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Classifications MeSH