Concentration Determination of >200 Proteins in Dried Blood Spots for Biomarker Discovery and Validation.
HPLC
Multiple reaction monitoring
biofluids
biomarker: diagnostic
biomarker: prognostic
blood
dried blood spot
high throughput screening
mass spectrometry
omics
quantification
Journal
Molecular & cellular proteomics : MCP
ISSN: 1535-9484
Titre abrégé: Mol Cell Proteomics
Pays: United States
ID NLM: 101125647
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
10
10
2019
revised:
06
12
2019
pubmed:
4
1
2020
medline:
5
11
2020
entrez:
4
1
2020
Statut:
ppublish
Résumé
The use of protein biomarkers as surrogates for clinical endpoints requires extensive multilevel validation including development of robust and sensitive assays for precise measurement of protein concentration. Multiple reaction monitoring (MRM) is a well-established mass-spectrometric method that can be used for reproducible protein-concentration measurements in biological specimens collected via microsampling. The dried blood spot (DBS) microsampling technique can be performed non-invasively without the expertise of a phlebotomist, and can enhance analyte stability which facilitate the application of this technique in retrospective studies while providing lower storage and shipping costs, because cold-chain logistics can be eliminated. Thus, precise, sensitive, and multiplexed methods for measuring protein concentrations in DBSs can be used for
Identifiants
pubmed: 31896676
pii: S1535-9476(20)35040-4
doi: 10.1074/mcp.TIR119.001820
pmc: PMC7050112
pii:
doi:
Substances chimiques
Biomarkers
0
Blood Proteins
0
Peptides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
540-553Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2020 Eshghi et al.
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