MAP3Kinase-dependent SnRK2-kinase activation is required for abscisic acid signal transduction and rapid osmotic stress response.
Abscisic Acid
/ metabolism
Animals
Arabidopsis
/ physiology
Arabidopsis Proteins
/ genetics
Female
Mutation
Oocytes
/ metabolism
Osmotic Pressure
/ physiology
Phosphorylation
Plants, Genetically Modified
Protein Kinases
/ genetics
Protein Serine-Threonine Kinases
/ genetics
Serine
/ metabolism
Signal Transduction
Xenopus laevis
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
02 01 2020
02 01 2020
Historique:
received:
29
05
2019
accepted:
04
12
2019
entrez:
4
1
2020
pubmed:
4
1
2020
medline:
14
4
2020
Statut:
epublish
Résumé
Abiotic stresses, including drought and salinity, trigger a complex osmotic-stress and abscisic acid (ABA) signal transduction network. The core ABA signalling components are snf1-related protein kinase2s (SnRK2s), which are activated by ABA-triggered inhibition of type-2C protein-phosphatases (PP2Cs). SnRK2 kinases are also activated by a rapid, largely unknown, ABA-independent osmotic-stress signalling pathway. Here, through a combination of a redundancy-circumventing genetic screen and biochemical analyses, we have identified functionally-redundant MAPKK-kinases (M3Ks) that are necessary for activation of SnRK2 kinases. These M3Ks phosphorylate a specific SnRK2/OST1 site, which is indispensable for ABA-induced reactivation of PP2C-dephosphorylated SnRK2 kinases. ABA-triggered SnRK2 activation, transcription factor phosphorylation and SLAC1 activation require these M3Ks in vitro and in plants. M3K triple knock-out plants show reduced ABA sensitivity and strongly impaired rapid osmotic-stress-induced SnRK2 activation. These findings demonstrate that this M3K clade is required for ABA- and osmotic-stress-activation of SnRK2 kinases, enabling robust ABA and osmotic stress signal transduction.
Identifiants
pubmed: 31896774
doi: 10.1038/s41467-019-13875-y
pii: 10.1038/s41467-019-13875-y
pmc: PMC6940395
doi:
Substances chimiques
Arabidopsis Proteins
0
SnRK2 protein, Arabidopsis
0
Serine
452VLY9402
Abscisic Acid
72S9A8J5GW
Protein Kinases
EC 2.7.-
OST1 protein, Arabidopsis
EC 2.7.1.-
Protein Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
12Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM060396
Pays : United States
Organisme : NIEHS NIH HHS
ID : P42 ES010337
Pays : United States
Organisme : NIH HHS
ID : S10 OD021724
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM114660
Pays : United States
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