Direct single-molecule quantification reveals unexpectedly high mechanical stability of vinculin-talin/α-catenin linkages.
Actin Cytoskeleton
/ metabolism
Binding Sites
Cell Adhesion
/ physiology
Focal Adhesions
/ metabolism
Humans
Integrins
/ metabolism
Mechanotransduction, Cellular
/ physiology
Plasmids
/ genetics
Protein Binding
Single Molecule Imaging
/ methods
Talin
/ metabolism
Vinculin
/ chemistry
alpha Catenin
/ metabolism
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
31
08
2018
accepted:
29
10
2019
entrez:
4
1
2020
pubmed:
4
1
2020
medline:
22
7
2020
Statut:
epublish
Résumé
The vinculin-mediated mechanosensing requires establishment of stable mechanical linkages between vinculin to integrin at focal adhesions and to cadherins at adherens junctions through associations with the respective adaptor proteins talin and α-catenin. However, the mechanical stability of these critical vinculin linkages has yet to be determined. Here, we developed a single-molecule detector assay to provide direct quantification of the mechanical lifetime of vinculin association with the vinculin binding sites in both talin and α-catenin, which reveals a surprisingly high mechanical stability of the vinculin-talin and vinculin-α-catenin interfaces that have a lifetime of >1000 s at forces up to 10 pN and can last for seconds to tens of seconds at 15 to 25 pN. Our results suggest that these force-bearing intermolecular interfaces provide sufficient mechanical stability to support the vinculin-mediated mechanotransduction at cell-matrix and cell-cell adhesions.
Identifiants
pubmed: 31897422
doi: 10.1126/sciadv.aav2720
pii: aav2720
pmc: PMC6920023
doi:
Substances chimiques
CTNNA1 protein, human
0
CTNNAL1 protein, human
0
Integrins
0
TLN1 protein, human
0
Talin
0
VCL protein, human
0
alpha Catenin
0
Vinculin
125361-02-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
eaav2720Informations de copyright
Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
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