Conditional control of RNA-guided nucleic acid cleavage and gene editing.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
03 01 2020
03 01 2020
Historique:
received:
03
01
2019
accepted:
20
11
2019
entrez:
5
1
2020
pubmed:
5
1
2020
medline:
25
3
2020
Statut:
epublish
Résumé
Prokaryotes use repetitive genomic elements termed CRISPR (clustered regularly interspaced short palindromic repeats) to destroy invading genetic molecules. Although CRISPR systems have been widely used in DNA and RNA technology, certain adverse effects do occur. For example, constitutively active CRISPR systems may lead to a certain risk of off-target effects. Here, we introduce post-synthetic masking and chemical activation of guide RNA (gRNA) to controlling CRISPR systems. An RNA structure profiling probe (2-azidomethylnicotinic acid imidazolide) is used. Moreover, we accomplish conditional control of gene editing in live cells. This proof-of-concept study demonstrates promising potential of chemical activation of gRNAs as a versatile tool for chemical biology.
Identifiants
pubmed: 31900392
doi: 10.1038/s41467-019-13765-3
pii: 10.1038/s41467-019-13765-3
pmc: PMC6941951
doi:
Substances chimiques
RNA, Guide
0
RNA
63231-63-0
Endonucleases
EC 3.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
91Références
Nat Biotechnol. 2013 Sep;31(9):839-43
pubmed: 23934178
Cell Rep. 2015 Mar 3;10(8):1422-32
pubmed: 25732831
Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):E11257-E11266
pubmed: 29229813
Science. 2016 Aug 5;353(6299):aaf5573
pubmed: 27256883
Nat Chem. 2014 Apr;6(4):352-61
pubmed: 24651204
J Biotechnol. 2015 Aug 20;208:44-53
pubmed: 26003884
Mol Cell. 2015 Nov 5;60(3):385-97
pubmed: 26593719
Angew Chem Int Ed Engl. 2018 Mar 12;57(12):3059-3063
pubmed: 29370460
Hum Gene Ther. 2016 Jun;27(6):464-75
pubmed: 27094534
J Exp Med. 2018 Mar 5;215(3):985-997
pubmed: 29436394
Angew Chem Int Ed Engl. 2016 Sep 26;55(40):12440-4
pubmed: 27554600
Nature. 2014 May 22;509(7501):487-91
pubmed: 24717434
Nanoscale. 2018 Jan 18;10(3):1063-1071
pubmed: 29266160
Front Physiol. 2017 Apr 28;8:256
pubmed: 28503153
Proc Natl Acad Sci U S A. 2012 Sep 25;109(39):E2579-86
pubmed: 22949671
J Am Chem Soc. 2015 May 6;137(17):5642-5
pubmed: 25905628
Cell Stem Cell. 2015 Aug 6;17(2):233-44
pubmed: 26145478
Angew Chem Int Ed Engl. 2018 Mar 5;57(11):2768-2798
pubmed: 28521066
Nature. 2015 Mar 26;519(7544):486-90
pubmed: 25799993
Genome Res. 2014 Jun;24(6):1012-9
pubmed: 24696461
Proc Natl Acad Sci U S A. 2015 Sep 22;112(38):11870-5
pubmed: 26324940
Sci Adv. 2019 Apr 03;5(4):eaav7199
pubmed: 30949579
Nat Biotechnol. 2015 Jul;33(7):755-60
pubmed: 26076431
Science. 2007 Mar 23;315(5819):1709-12
pubmed: 17379808
Cell. 2017 Aug 10;170(4):714-726.e10
pubmed: 28757251
J Am Chem Soc. 2018 Mar 14;140(10):3491-3495
pubmed: 29474085
J Am Chem Soc. 2017 Mar 15;139(10):3790-3795
pubmed: 28218837
Nature. 2016 Oct 13;538(7624):270-273
pubmed: 27669025
Mol Cell. 2016 Aug 4;63(3):355-70
pubmed: 27494557
Cell. 2014 Feb 27;156(5):935-49
pubmed: 24529477
Nat Biotechnol. 2015 Feb;33(2):139-42
pubmed: 25643054
Nat Chem Biol. 2015 May;11(5):316-8
pubmed: 25848930
Nat Chem Biol. 2016 Nov;12(11):980-987
pubmed: 27618190
Science. 2017 Nov 24;358(6366):1019-1027
pubmed: 29070703
Nucleic Acids Res. 2015 Jul 27;43(13):6450-8
pubmed: 26082496
Nat Biotechnol. 2017 Dec;35(12):1179-1187
pubmed: 29131148
Nucleic Acids Res. 2016 Nov 2;44(19):e149
pubmed: 27458201
Nat Chem. 2016 Nov;8(11):1027-1034
pubmed: 27768095
Org Lett. 2014 Jan 3;16(1):30-3
pubmed: 24299163
Nucleic Acids Res. 2011 Nov;39(21):9275-82
pubmed: 21813460
Science. 2016 Jan 1;351(6268):84-8
pubmed: 26628643
Nature. 2010 Nov 4;468(7320):67-71
pubmed: 21048762
J Am Chem Soc. 2018 Jun 6;140(22):6741-6744
pubmed: 29792692
Science. 2012 Aug 17;337(6096):816-21
pubmed: 22745249
Nat Commun. 2016 Nov 02;7:13350
pubmed: 27804953
Nat Biotechnol. 2015 Sep;33(9):985-989
pubmed: 26121415
Science. 2017 Apr 28;356(6336):438-442
pubmed: 28408723
Nature. 2017 Oct 19;550(7676):407-410
pubmed: 28931002
Nat Chem. 2015 Jul;7(7):554-561
pubmed: 26100803
Nat Biotechnol. 2013 Sep;31(9):822-6
pubmed: 23792628
Protein Cell. 2015 May;6(5):363-372
pubmed: 25894090
Science. 2013 Feb 15;339(6121):823-6
pubmed: 23287722
Nature. 2011 Mar 31;471(7340):602-7
pubmed: 21455174
Nat Biotechnol. 2015 Apr;33(4):390-394
pubmed: 25690852
Front Mol Neurosci. 2016 Aug 18;9:70
pubmed: 27587996
Angew Chem Int Ed Engl. 2015 Apr 7;54(15):4597-602
pubmed: 25694369
Science. 2013 Feb 15;339(6121):819-23
pubmed: 23287718
Cell Stem Cell. 2014 Aug 7;15(2):215-226
pubmed: 24931489
Nat Chem Biol. 2015 Mar;11(3):198-200
pubmed: 25664691
Nat Commun. 2018 Apr 13;9(1):1448
pubmed: 29654299