Evolutionarily conserved pachytene piRNA loci are highly divergent among modern humans.
Journal
Nature ecology & evolution
ISSN: 2397-334X
Titre abrégé: Nat Ecol Evol
Pays: England
ID NLM: 101698577
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
10
07
2019
accepted:
19
11
2019
entrez:
5
1
2020
pubmed:
5
1
2020
medline:
31
3
2020
Statut:
ppublish
Résumé
In the fetal mouse testis, PIWI-interacting RNAs (piRNAs) guide PIWI proteins to silence transposons but, after birth, most post-pubertal pachytene piRNAs map to the genome uniquely and are thought to regulate genes required for male fertility. In the human male, the developmental classes, precise genomic origins and transcriptional regulation of postnatal piRNAs remain undefined. Here, we demarcate the genes and transcripts that produce postnatal piRNAs in human juvenile and adult testes. As in the mouse, human A-MYB drives transcription of both pachytene piRNA precursor transcripts and messenger RNAs encoding piRNA biogenesis factors. Although human piRNA genes are syntenic to those in other placental mammals, their sequences are poorly conserved. In fact, pachytene piRNA loci are rapidly diverging even among modern humans. Our findings suggest that, during mammalian evolution, pachytene piRNA genes are under few selective constraints. We speculate that pachytene piRNA diversity may provide a hitherto unrecognized driver of reproductive isolation.
Identifiants
pubmed: 31900453
doi: 10.1038/s41559-019-1065-1
pii: 10.1038/s41559-019-1065-1
pmc: PMC6961462
mid: NIHMS1544104
doi:
Substances chimiques
RNA, Messenger
0
RNA, Small Interfering
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
156-168Subventions
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NICHD NIH HHS
ID : P01 HD078253
Pays : United States
Organisme : NIGMS NIH HHS
ID : R37 GM062862
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001453
Pays : United States
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