Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging.
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
01
05
2019
accepted:
11
11
2019
pubmed:
8
1
2020
medline:
6
5
2020
entrez:
8
1
2020
Statut:
ppublish
Résumé
Combining the molecular specificity of fluorescent probes with three-dimensional imaging at nanoscale resolution is critical for investigating the spatial organization and interactions of cellular organelles and protein complexes. We present a 4Pi single-molecule switching super-resolution microscope that enables ratiometric multicolor imaging of mammalian cells at 5-10-nm localization precision in three dimensions using 'salvaged fluorescence'. Imaging two or three fluorophores simultaneously, we show fluorescence images that resolve the highly convoluted Golgi apparatus and the close contacts between the endoplasmic reticulum and the plasma membrane, structures that have traditionally been the imaging realm of electron microscopy. The salvaged fluorescence approach is equally applicable in most single-objective microscopes.
Identifiants
pubmed: 31907447
doi: 10.1038/s41592-019-0676-4
pii: 10.1038/s41592-019-0676-4
pmc: PMC7028321
mid: NIHMS1542660
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
225-231Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM118486
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK045735
Pays : United States
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NINDS NIH HHS
ID : R37 NS036251
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS036251
Pays : United States
Organisme : Wellcome Trust
ID : 203285/Z/16/Z
Pays : United Kingdom
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