Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping.
Apolipoprotein E
HDL, high density lipoprotein
IDL, intermediate density lipoprotein
IEF, isoelectric focusing
Isoelectric focusing
Isoforms
LDL, low density lipoprotein
Phenotype
VLDL, very low density lipoprotein
apo, apolipoprotein
Journal
Practical laboratory medicine
ISSN: 2352-5517
Titre abrégé: Pract Lab Med
Pays: Netherlands
ID NLM: 101690848
Informations de publication
Date de publication:
Jan 2020
Jan 2020
Historique:
received:
29
08
2019
revised:
08
12
2019
accepted:
11
12
2019
entrez:
8
1
2020
pubmed:
8
1
2020
medline:
8
1
2020
Statut:
epublish
Résumé
A qualitative, semi-automatized method for apolipoprotein E (apoE) phenotyping by isoelectric focusing method has been evaluated on 40 serum samples from patients previously genotyped for apoE, especially as regards concordance with genotyping, but also repeatability and reproducibility of the method, and sample storage. Total concordance with genotyping and good precision criteria, together with its practicability and requirement of a little sample volume, lead to conclude to the usefulness of this method to help clinicians in the diagnosis of dyslipidemic and neurodegenerative diseases.
Identifiants
pubmed: 31909147
doi: 10.1016/j.plabm.2019.e00150
pii: S2352-5517(19)30107-6
pii: e00150
pmc: PMC6939034
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e00150Informations de copyright
© 2019 The Authors.
Déclaration de conflit d'intérêts
There are no known conflicts of interest.
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