Hypoxia-inducible factor 1α promotes interleukin 1β and tumour necrosis factor α expression in lipopolysaccharide-stimulated human dental pulp cells.
HIF1α
NFκB signalling
human dental pulp cells
lipopolysaccharide
pro-inflammatory cytokine
Journal
International endodontic journal
ISSN: 1365-2591
Titre abrégé: Int Endod J
Pays: England
ID NLM: 8004996
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
07
05
2019
accepted:
31
12
2019
pubmed:
8
1
2020
medline:
15
4
2020
entrez:
8
1
2020
Statut:
ppublish
Résumé
To elucidate the role of HIF1α in pro-inflammatory cytokine mRNA expression from lipopolysaccharide (LPS)-stimulated human dental pulp cells (hDPCs). mRNA expression of interleukin (IL) 1β and tumour necrosis factor (TNF) α in LPS-stimulated hDPCs was determined by quantitative RT-PCR. Expression of nuclear factor kappa B (NFκB) p65 and phospho-NFκB p65 was analysed by Western blotting. Activation of NFκB signalling was measured by luciferase assay using a reporter vector containing an NFκB response element. Enforced expression of HIF1α was induced by transfection of expression vectors with native or constitutively active forms of HIF1α. Expression of HIF1α protein in hDPCs was evaluated by immunocytochemistry and Western blotting. One-way analysis of variance and the Tukey-Kramer test were performed to determine a significant difference (P < 0.05). mRNA expression of IL1β and TNFα, protein expression of phospho-NFκB p65 and LPS-induced NFκB signalling activity were promoted in low oxygen conditions (1% O HIF1α promoted mRNA expression of IL1β and TNFα via NFκB signalling in LPS-stimulated hDPCs, suggesting that HIF1α is involved in the progress of inflammation in dental pulp.
Substances chimiques
Interleukin-1beta
0
Lipopolysaccharides
0
NF-kappa B
0
Tumor Necrosis Factor-alpha
0
Types de publication
Journal Article
Langues
eng
Pagination
636-646Subventions
Organisme : Japan Society for the Promotion of Science
ID : 17H04380
Informations de copyright
© 2020 International Endodontic Society. Published by John Wiley & Sons Ltd.
Références
Chandel NS, Trzyna WC, McClintock DS, Schumacker PT (2000) Role of oxidants in NF-kappa B activation and TNF-alpha gene transcription induced by hypoxia and endotoxin. Journal of Immunology 165, 1013-21.
Chang J, Zhang C, Tani-Ishii N, Shi S, Wang CY (2005) NF-kappaB activation in human dental pulp stem cells by TNF and LPS. Journal of Dental Research 84, 994-8.
Chow JC, Young DW, Golenbock DT, Christ WJ, Gusovsky F (1999) Toll-like receptor-4 mediates lipopolysaccharide-induced signal transduction. Journal of Biological Chemistry 274, 10689-92.
Cooper PR, Smith AJ (2013) Molecular mediators of pulp inflammation and regeneration. Endodontic Topics 28, 90-105.
Cramer T, Yamanishi Y, Clausen BE et al. (2003) HIF-1alpha is essential for myeloid cell-mediated inflammation. Cell 112, 645-57.
Cummins EP, Berra E, Comerford KM et al. (2006) Prolyl hydroxylase-1 negatively regulates IkappaB kinase-beta, giving insight into hypoxia-induced NFkappaB activity. Proceedings of the National Academy of Sciences of the United States of America 103, 18154-9.
Dengler VL, Galbraith M, Espinosa JM (2014) Transcriptional regulation by hypoxia inducible factors. Critical Reviews in Biochemistry and Molecular Biology 49, 1-15.
Devignes CS, Aslan Y, Brenot A et al. (2018) HIF signaling in osteoblast-lineage cells promotes systemic breast cancer growth and metastasis in mice. Proceedings of the National Academy of Sciences of the United States of America 115, E992-E1001.
Eltzschig HK, Carmeliet P (2011) Hypoxia and inflammation. New England Journal of Medicine 364, 656-65.
Hahn CL, Liewehr FR (2007) Innate immune responses of the dental pulp to caries. Journal of Endodontics 33, 643-51.
Heyeraas KJ, Berggreen E (1999) Interstitial fluid pressure in normal and inflamed pulp. Critical Reviews in Oral Biology and Medicine 10, 328-36.
Hoesel B, Schmid JA (2013) The complexity of NF-κB signaling in inflammation and cancer. Molecular Cancer 12, 86.
Hollander AP, Corke KP, Freemont AJ, Lewis CE (2001) Expression of hypoxia-inducible factor 1 alpha by macrophages in the rheumatoid synovium: implications for targeting of therapeutic genes to the inflamed joint. Arthritis and Rheumatism 44, 1540-4.
Ivan M, Kondo K, Yang H et al. (2001) HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: Implications for O2 sensing. Science 292, 464-8.
Jaakkola P, Mole DR, Tian YM et al. (2001) Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation. Science 292, 468-72.
Jontell M, Okiji T, Dahlgren U, Bergenholtz G (1998) Immune defense mechanisms of the dental pulp. Critical Reviews in Oral Biology and Medicine 9, 179-200.
Kaelin WG Jr, Ratcliffe PJ (2008) Oxygen sensing by metazoans: the central role of the HIF hydroxylase pathway. Molecular Cell 30, 393-402.
Kamal AM, Okiji T, Kawashima N, Suda H (1997) Defense responses of dentin/pulp complex to experimentally induced caries in rat molars: an immunohistochemical study on kinetics of pulpal Ia antigen-expressing cells and macrophages. Journal of Endodontics 23, 115-20.
Kawashima N, Okiji T (2016) Odontoblasts: Specialized hard-tissue-forming cells in the dentin-pulp complex. Congenital Anomalies 56, 144-53.
Kawashima N, Noda S, Yamamoto M, Okiji T (2017) Properties of dental pulp-derived mesenchymal stem cells and the effects of culture conditions. Journal of Endodontics 43, S31-4.
Kondo K, Klco J, Nakamura E, Lechpammer M, Kaelin WG Jr (2002) Inhibition of HIF is necessary for tumor suppression by the von Hippel-Lindau protein. Cancer Cell 1, 237-46.
Koong AC, Chen EY, Giaccia AJ (1994) Hypoxia causes the activation of nuclear kappa B through the phosphorylation of I kappa B alpha on tyrosine residues. Cancer Research 54, 1425-30.
Lawrence T (2009) The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harbor Perspectives in Biology 1, a001651.
Liu FQ, Liu Y, Lui VC, Lamb JR, Tam PK, Chen Y (2008) Hypoxia modulates lipopolysaccharide induced TNF-alpha expression in murine macrophages. Experimental Cell Research 314, 1327-36.
Majmundar AJ, Wong WJ, Simon MC (2010) Hypoxia-inducible factors and the response to hypoxic stress. Molecular Cell 40, 294-309.
Mutoh N, Tani-Ishii N, Tsukinoki K, Chieda K, Watanabe K (2007) Expression of toll-like receptor 2 and 4 in dental pulp. Journal of Endodontics 33, 1183-6.
Okiji T, Kawashima N, Kosaka T, Matsumoto A, Kobayashi C, Suda H (1992) An immunohistochemical study of the distribution of immunocompetent cells, especially macrophages and Ia antigen-expressing cells of heterogeneous populations, in normal rat molar pulp. Journal of Dental Research 71, 1196-202.
Peyssonnaux C, Cejudo-Martin P, Doedens A, Zinkernagel AS, Johnson RS, Nizet V (2007) Cutting edge: essential role of hypoxia inducible factor-1alpha in development of lipopolysaccharide-induced sepsis. Journal of Immunology 178, 7516-9.
Ratcliffe PJ (2013) Oxygen sensing and hypoxia signaling pathways in animals: the implications of physiology for cancer. Journal of Physiology 591, 2077-42.
Rey S, Semenza GL (2010) Hypoxia-inducible factor-1-dependent mechanisms of vascularization and vascular remodelling. Cardiovascular Research 86, 236-42.
Suda T, Takubo K, Semenza GL (2011) Metabolic regulation of hematopoietic stem cells in the hypoxic niche. Cell Stem Cell 9, 298-310.
Taylor CT (2008) Interdependent roles for hypoxia inducible factor and nuclear factor-kappaB in hypoxic inflammation. Journal of Physiology 586, 4055-9.
Walmsley SR, Print C, Farahi N et al. (2005) Hypoxia-induced neutrophil survival is mediated by HIF-1alpha-dependent NF-kappaB activity. Journal of Experimental Medicine 201, 105-15.
Yan Q, Bartz S, Mao M, Li L, Kaelin WG Jr (2007) The hypoxia-inducible factor 2alpha N-terminal and C-terminal transactivation domains cooperate to promote renal tumorigenesis in vivo. Molecular and Cellular Biology 27, 2092-102.
Yoshida T, Hashimura M, Mastumoto T et al. (2013) Transcriptional upregulation of HIF-1α by NF-κB/p65 and its associations with β-catenin/p300 complexes in endometrial carcinoma cells. Laboratory Investigation 93, 1184-93.
Yu CY, Boyd NM, Cringle SJ, Alder VA, Yu DY (2002) Oxygen distribution and consumption in rat lower incisor pulp. Archives of Oral Biology 47, 529-36.