An adipose tissue galectin controls endothelial cell function via preferential recognition of 3-fucosylated glycans.
Adipocytes
/ metabolism
Adipose Tissue
/ metabolism
Animals
Cell Physiological Phenomena
/ physiology
Endothelial Cells
/ metabolism
Galectins
/ metabolism
Insulin Resistance
/ physiology
Lipid Droplets
/ metabolism
Lipolysis
/ physiology
Mice, Knockout
Neovascularization, Pathologic
/ metabolism
Polysaccharides
/ metabolism
adipose tissue
angiogenesis
galectins
glycosylation
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
19
07
2019
revised:
08
10
2019
accepted:
21
10
2019
entrez:
10
1
2020
pubmed:
10
1
2020
medline:
8
7
2020
Statut:
ppublish
Résumé
Upon overnutrition, adipocytes activate a homeostatic program to adjust anabolic pressure. An inflammatory response enables adipose tissue (AT) expansion with concomitant enlargement of its capillary network, and reduces energy storage by increasing insulin resistance. Galectin-12 (Gal-12), an endogenous lectin preferentially expressed in AT, plays a key role in adipocyte differentiation, lipolysis, and glucose homeostasis. Here, we reveal biochemical and biophysical determinants of Gal-12 structure, including its preferential recognition of 3-fucosylated structures, a unique feature among members of the galectin family. Furthermore, we identify a previously unanticipated role for this lectin in the regulation of angiogenesis within AT. Gal-12 showed preferential localization within the inner side of lipid droplets, and its expression was upregulated under hypoxic conditions. Through glycosylation-dependent binding to endothelial cells, Gal-12 promoted in vitro angiogenesis. Moreover, analysis of in vivo AT vasculature showed reduced vascular networks in Gal-12-deficient (Lgals12
Identifiants
pubmed: 31914594
doi: 10.1096/fj.201901817R
doi:
Substances chimiques
Galectins
0
Polysaccharides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
735-753Informations de copyright
© 2019 Federation of American Societies for Experimental Biology.
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