Acute environmental hypoxia potentiates satellite cell-dependent myogenesis in response to resistance exercise through the inflammation pathway in human.
Cells, Cultured
Exercise
/ physiology
Humans
Hypoxia
/ metabolism
Inflammation
/ metabolism
Muscle Development
/ physiology
Muscle Fibers, Skeletal
/ metabolism
Muscle Proteins
/ metabolism
Muscle, Skeletal
/ metabolism
RNA, Messenger
/ metabolism
Resistance Training
/ methods
Satellite Cells, Skeletal Muscle
/ metabolism
Signal Transduction
/ physiology
exercise
hypoxia
inflammation
muscle
myogenesis
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
03
09
2019
revised:
31
10
2019
accepted:
21
11
2019
entrez:
10
1
2020
pubmed:
10
1
2020
medline:
7
7
2020
Statut:
ppublish
Résumé
Acute environmental hypoxia may potentiate muscle hypertrophy in response to resistance training but the mechanisms are still unknown. To this end, twenty subjects performed a 1-leg knee extension session (8 sets of 8 repetitions at 80% 1 repetition maximum, 2-min rest between sets) in normoxic or normobaric hypoxic conditions (FiO2 14%). Muscle biopsies were taken 15 min and 4 hours after exercise in the vastus lateralis of the exercised and the non-exercised legs. Blood samples were taken immediately, 2h and 4h after exercise. In vivo, hypoxic exercise fostered acute inflammation mediated by the TNFα/NF-κB/IL-6/STAT3 (+333%, +194%, + 163% and +50% respectively) pathway, which has been shown to contribute to satellite cells myogenesis. Inflammation activation was followed by skeletal muscle invasion by CD68 (+63%) and CD197 (+152%) positive immune cells, both known to regulate muscle regeneration. The role of hypoxia-induced activation of inflammation in myogenesis was confirmed in vitro. Acute hypoxia promoted myogenesis through increased Myf5 (+300%), MyoD (+88%), myogenin (+1816%) and MRF4 (+489%) mRNA levels in primary myotubes and this response was blunted by siRNA targeting STAT3. In conclusion, our results suggest that hypoxia could improve muscle hypertrophic response following resistance exercise through IL-6/STAT3-dependent myogenesis and immune cells-dependent muscle regeneration.
Identifiants
pubmed: 31914659
doi: 10.1096/fj.201902244R
doi:
Substances chimiques
Muscle Proteins
0
RNA, Messenger
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1885-1900Informations de copyright
© 2019 Federation of American Societies for Experimental Biology.
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