Circadian disruption by shifting the light-dark cycle negatively affects bone health in mice.
ARNTL Transcription Factors
/ metabolism
Animals
Behavior, Animal
Bone Density
Bone and Bones
/ physiology
CLOCK Proteins
/ metabolism
Cathepsin K
/ metabolism
Circadian Clocks
Circadian Rhythm
Cryptochromes
/ metabolism
Female
Gene Expression Regulation
Light
Lipids
/ chemistry
Mice
Nuclear Receptor Subfamily 1, Group D, Member 1
/ metabolism
Osteogenesis
Osteoprotegerin
/ metabolism
Period Circadian Proteins
/ metabolism
Photoperiod
Proto-Oncogene Proteins c-fos
/ metabolism
RANK Ligand
/ metabolism
X-Ray Microtomography
bone mineralization
bone structure
bone turnover
circadian rhythm
clock genes
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
30
07
2019
revised:
16
10
2019
accepted:
04
11
2019
entrez:
10
1
2020
pubmed:
10
1
2020
medline:
21
7
2020
Statut:
ppublish
Résumé
The past decade, it has become evident that circadian rhythms within metabolically active tissues are very important for physical health. However, although shift work has also been associated with an increased risk of fractures, circadian rhythmicity has not yet been extensively studied in bone. Here, we investigated which genes are rhythmically expressed in bone, and whether circadian disruption by shifts in light-dark cycle affects bone turnover and structure in mice. Our results demonstrate diurnal expression patterns of clock genes (Rev-erbα, Bmal1, Per1, Per2, Cry1, Clock), as well as genes involved in osteoclastogenesis, osteoclast proliferation and function (Rankl, Opg, Ctsk), and osteocyte function (c-Fos) in bone. Weekly alternating light-dark cycles disrupted rhythmic clock gene expression in bone and caused a reduction in plasma levels of procollagen type 1 amino-terminal propeptide (P1NP) and tartrate-resistant acidic phosphatase (TRAP), suggestive of a reduced bone turnover. These effects coincided with an altered trabecular bone structure and increased cortical mineralization after 15 weeks of light-dark cycles, which may negatively affect bone strength in the long term. Collectively, these results show that a physiological circadian rhythm is important to maintain bone health, which stresses the importance of further investigating the association between shift work and skeletal disorders.
Identifiants
pubmed: 31914701
doi: 10.1096/fj.201901929R
doi:
Substances chimiques
ARNTL Transcription Factors
0
Bmal1 protein, mouse
0
Cry1 protein, mouse
0
Cryptochromes
0
Lipids
0
Nr1d1 protein, mouse
0
Nuclear Receptor Subfamily 1, Group D, Member 1
0
Osteoprotegerin
0
Per1 protein, mouse
0
Per2 protein, mouse
0
Period Circadian Proteins
0
Proto-Oncogene Proteins c-fos
0
RANK Ligand
0
Tnfrsf11b protein, mouse
0
Tnfsf11 protein, mouse
0
CLOCK Proteins
EC 2.3.1.48
Clock protein, mouse
EC 2.3.1.48
Cathepsin K
EC 3.4.22.38
Ctsk protein, mouse
EC 3.4.22.38
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1052-1064Informations de copyright
© 2019 The Authors. The FASEB Journal published by Wiley Periodicals, Inc. on behalf of Federation of American Societies for Experimental Biology.
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