Development and Evaluation of Modified Cryopreservation for Long-Term Storage of Blastocystis Subtypes 1-3 and 6.
Blastocystis
Cryopreservation
Long-term storage
Subtypes
Journal
Acta parasitologica
ISSN: 1896-1851
Titre abrégé: Acta Parasitol
Pays: Switzerland
ID NLM: 9301947
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
05
08
2019
accepted:
05
12
2019
pubmed:
11
1
2020
medline:
4
3
2021
entrez:
11
1
2020
Statut:
ppublish
Résumé
Blastocystis is a protozoan parasite living in the intestine of humans and a wide range of animals. Although Blastocystis grows in several cultivation media, axenification and serial cultivations for long time are the main challenges of the researchers. Therefore, the long-term storage of subtypes/strains of Blastocystis using cryopreservation provides a suitable source of this parasite for the physiological, biochemical, and biological studies. In the current study, seven xenic isolates including two separated isolates from ST1-3 and one isolate from ST6 were cryopreserved using a standard method with minor modifications. After 3 months, all isolates were recovered and cultivated in DMEM medium. The findings of the method showed all seven isolates were successfully recovered in DMEM medium. In addition, all isolates remained viable after several sub-cultures. It seems that cryopreservation is a simple method that can provide a suitable condition for the long-term storage of Blastocystis.
Sections du résumé
BACKGROUND
BACKGROUND
Blastocystis is a protozoan parasite living in the intestine of humans and a wide range of animals. Although Blastocystis grows in several cultivation media, axenification and serial cultivations for long time are the main challenges of the researchers. Therefore, the long-term storage of subtypes/strains of Blastocystis using cryopreservation provides a suitable source of this parasite for the physiological, biochemical, and biological studies.
METHODS
METHODS
In the current study, seven xenic isolates including two separated isolates from ST1-3 and one isolate from ST6 were cryopreserved using a standard method with minor modifications. After 3 months, all isolates were recovered and cultivated in DMEM medium.
RESULTS
RESULTS
The findings of the method showed all seven isolates were successfully recovered in DMEM medium. In addition, all isolates remained viable after several sub-cultures.
CONCLUSIONS
CONCLUSIONS
It seems that cryopreservation is a simple method that can provide a suitable condition for the long-term storage of Blastocystis.
Identifiants
pubmed: 31919797
doi: 10.2478/s11686-019-00157-2
pii: 10.2478/s11686-019-00157-2
doi:
Substances chimiques
Nitrogen
N762921K75
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM