Single Cell and Single Nucleus RNA-Seq Reveal Cellular Heterogeneity and Homeostatic Regulatory Networks in Adult Mouse Stria Vascularis.
EP
adult stria vascularis
hearing
regulons
scRNA-Seq
snRNA-Seq
Journal
Frontiers in molecular neuroscience
ISSN: 1662-5099
Titre abrégé: Front Mol Neurosci
Pays: Switzerland
ID NLM: 101477914
Informations de publication
Date de publication:
2019
2019
Historique:
received:
02
10
2019
accepted:
05
12
2019
entrez:
11
1
2020
pubmed:
11
1
2020
medline:
11
1
2020
Statut:
epublish
Résumé
The stria vascularis (SV) generates the endocochlear potential (EP) in the inner ear and is necessary for proper hair cell mechanotransduction and hearing. While channels belonging to SV cell types are known to play crucial roles in EP generation, relatively little is known about gene regulatory networks that underlie the ability of the SV to generate and maintain the EP. Using single cell and single nucleus RNA-sequencing, we identify and validate known and rare cell populations in the SV. Furthermore, we establish a basis for understanding molecular mechanisms underlying SV function by identifying potential gene regulatory networks as well as druggable gene targets. Finally, we associate known deafness genes with adult SV cell types. This work establishes a basis for dissecting the genetic mechanisms underlying the role of the SV in hearing and will serve as a basis for designing therapeutic approaches to hearing loss related to SV dysfunction.
Identifiants
pubmed: 31920542
doi: 10.3389/fnmol.2019.00316
pmc: PMC6933021
doi:
Types de publication
Journal Article
Langues
eng
Pagination
316Subventions
Organisme : Intramural NIH HHS
ID : ZIA DC000088
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIC DC000086
Pays : United States
Informations de copyright
Copyright © 2019 Korrapati, Taukulis, Olszewski, Pyle, Gu, Singh, Griffiths, Martin, Boger, Morell and Hoa.
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