Efficient and Robust NK-Cell Transduction With Baboon Envelope Pseudotyped Lentivector.
NK amplification and expansion system (NKAES)
NK-cell transduction
baboon retrovirus envelope pseudotyped lentivectors
chimeric antigen receptor
cytotoxicity
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2019
2019
Historique:
received:
05
07
2019
accepted:
22
11
2019
entrez:
11
1
2020
pubmed:
11
1
2020
medline:
5
11
2020
Statut:
epublish
Résumé
NK-cell resistance to transduction is a major technical hurdle for developing NK-cell immunotherapy. By using Baboon envelope pseudotyped lentiviral vectors (BaEV-LVs) encoding eGFP, we obtained a transduction rate of 23.0 ± 6.6% (mean ± SD) in freshly-isolated human NK-cells (FI-NK) and 83.4 ± 10.1% (mean ± SD) in NK-cells obtained from the NK-cell Activation and Expansion System (NKAES), with a sustained transgene expression for at least 21 days. BaEV-LVs outperformed Vesicular Stomatitis Virus type-G (VSV-G)-, RD114- and Measles Virus (MV)- pseudotyped LVs (
Identifiants
pubmed: 31921138
doi: 10.3389/fimmu.2019.02873
pmc: PMC6927467
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2873Subventions
Organisme : CIHR
ID : PJT-156347
Pays : Canada
Organisme : CIHR
ID : 130246
Pays : Canada
Informations de copyright
Copyright © 2019 Colamartino, Lemieux, Bifsha, Nicoletti, Chakravarti, Sanz, Roméro, Selleri, Béland, Guiot, Tremblay-Laganière, Dicaire, Barreiro, Lee, Verhoeyen and Haddad.
Références
PLoS One. 2012;7(1):e30264
pubmed: 22279576
Comput Struct Biotechnol J. 2016 Sep 28;14:357-362
pubmed: 27761200
Nat Commun. 2016 Jul 27;7:12320
pubmed: 27460500
Front Immunol. 2019 Dec 16;10:2873
pubmed: 31921138
Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2129-34
pubmed: 10051606
BMC Res Notes. 2016 Jun 16;9:312
pubmed: 27306375
Cancer Res. 2009 May 1;69(9):4010-7
pubmed: 19383914
Nat Biotechnol. 2016 May;34(5):525-7
pubmed: 27043002
Nucleic Acids Res. 2015 Feb 18;43(3):1577-92
pubmed: 25605798
Blood. 2014 Aug 21;124(8):1221-31
pubmed: 24951430
J Virol. 2003 Mar;77(5):2936-45
pubmed: 12584318
Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17372-7
pubmed: 17090675
Blood. 2013 Feb 14;121(7):1165-74
pubmed: 23243285
Leukemia. 2018 Feb;32(2):520-531
pubmed: 28725044
Blood. 2002 Aug 1;100(3):823-32
pubmed: 12130492
Hum Gene Ther. 2017 Oct;28(10):897-913
pubmed: 28810809
J Gene Med. 2007 Jul;9(7):579-84
pubmed: 17533614
Front Immunol. 2019 Aug 22;10:2001
pubmed: 31507603
J Virol. 2004 Feb;78(3):1219-29
pubmed: 14722277
Blood Adv. 2017 Oct 24;1(23):2088-2104
pubmed: 29296856