Expression of MiR-335 and its target metalloproteinase genes: clinical significance in breast cancer.


Journal

Archives of physiology and biochemistry
ISSN: 1744-4160
Titre abrégé: Arch Physiol Biochem
Pays: England
ID NLM: 9510153

Informations de publication

Date de publication:
Jun 2022
Historique:
pubmed: 11 1 2020
medline: 31 5 2022
entrez: 11 1 2020
Statut: ppublish

Résumé

Early diagnosis of breast cancer decreases mortality rate; therefore, novel diagnostic methods are urgently required. In this study, authors aimed to investigate the role of serum-derived miR-335 in breast cancer, and the expression of matrix metalloproteinase-2 (MMP2) and matrix metalloproteinase-9 (MMP9) and evaluating their feasibility and clinical utility as biomarkers for the early detection of breast cancer. Blood samples were collected from a total of 210 individuals who were enrolled in this study. The participants were divided into newly diagnosed breast cancer patients ( MiR 335 expression level was down-regulated in primary breast cancer group as compared to benign breast group and healthy individuals with 98% and 94.9% sensitivity and specificity, respectively. MMP2 and MMP9 showed significantly higher expression levels in breast cancer group as compared to both benign and healthy group and reporting 92.7% and 93% sensitivity, respectively. The relations between investigated markers and pathologic types, staging, grading, and lymph node involvement were significant with these factors. Expression level of miR-335 was decreased with increased MMP2 and MMP9 at significant level. MiR-335, MMP2, and MMP9 can be used as diagnostic markers in breast cancer.

Sections du résumé

BACKGROUND UNASSIGNED
Early diagnosis of breast cancer decreases mortality rate; therefore, novel diagnostic methods are urgently required. In this study, authors aimed to investigate the role of serum-derived miR-335 in breast cancer, and the expression of matrix metalloproteinase-2 (MMP2) and matrix metalloproteinase-9 (MMP9) and evaluating their feasibility and clinical utility as biomarkers for the early detection of breast cancer.
MATERIALS AND METHODS UNASSIGNED
Blood samples were collected from a total of 210 individuals who were enrolled in this study. The participants were divided into newly diagnosed breast cancer patients (
RESULTS UNASSIGNED
MiR 335 expression level was down-regulated in primary breast cancer group as compared to benign breast group and healthy individuals with 98% and 94.9% sensitivity and specificity, respectively. MMP2 and MMP9 showed significantly higher expression levels in breast cancer group as compared to both benign and healthy group and reporting 92.7% and 93% sensitivity, respectively. The relations between investigated markers and pathologic types, staging, grading, and lymph node involvement were significant with these factors. Expression level of miR-335 was decreased with increased MMP2 and MMP9 at significant level.
CONCLUSION UNASSIGNED
MiR-335, MMP2, and MMP9 can be used as diagnostic markers in breast cancer.

Identifiants

pubmed: 31922434
doi: 10.1080/13813455.2019.1703004
doi:

Substances chimiques

MIRN335 microRNA, human 0
MicroRNAs 0
MMP2 protein, human EC 3.4.24.24
Matrix Metalloproteinase 2 EC 3.4.24.24
MMP9 protein, human EC 3.4.24.35
Matrix Metalloproteinase 9 EC 3.4.24.35

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

569-575

Auteurs

Amal Ramadan (A)

Biochemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Dokki, Giza, Egypt.
High Throughput Molecular and Genetic laboratory, Center for Excellences for Advanced Sciences, National Research Centre, Dokki, Egypt.

Maha Hashim (M)

Biochemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Dokki, Giza, Egypt.
High Throughput Molecular and Genetic laboratory, Center for Excellences for Advanced Sciences, National Research Centre, Dokki, Egypt.

Naglaa M Hassan (N)

Clinical Pathology Department, National Cancer Institute, Cairo, Egypt.

Menha Swellam (M)

Biochemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Dokki, Giza, Egypt.
High Throughput Molecular and Genetic laboratory, Center for Excellences for Advanced Sciences, National Research Centre, Dokki, Egypt.

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Classifications MeSH