Immunostimulatory effect of chitosan conjugated green copper oxide nanoparticles in tumor immunotherapy.
Adjuvants, Immunologic
/ pharmacology
Animals
CD4-Positive T-Lymphocytes
/ drug effects
Cell Line, Tumor
Cells, Cultured
Chitosan
/ immunology
Copper
/ immunology
Female
HeLa Cells
Humans
Immunotherapy
/ methods
MCF-7 Cells
Mice
Mice, Inbred BALB C
Nanoparticles
/ administration & dosage
Neoplasms
/ immunology
Adjuvant
Cytokines
IgG response
Macrophage activation
Surface coated nanoparticles
Journal
Cytokine
ISSN: 1096-0023
Titre abrégé: Cytokine
Pays: England
ID NLM: 9005353
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
28
08
2019
revised:
12
12
2019
accepted:
16
12
2019
pubmed:
11
1
2020
medline:
19
8
2021
entrez:
11
1
2020
Statut:
ppublish
Résumé
Current study demonstrates the immunogenic role of biopolymer coated green synthesized copper oxide nanoparticles by the induction of cellular immunity through the activation immune cells. Alongside humoral immunity response was triggered by the surface coated NPs through IgG response which indicate the adjuvanic role of the nano conjugate. Th1 (Type 1 and Type 2 helper T cells) and Th2 cells were activated after the treatment with nano conjugate and act as an immunostimulant which would inhibit the proliferation of breast cancer (MCF-7) and cervical cancer (HeLa) cells in in vitro. Solid tumor induced by 4 T1 cells were also inhibited in in vivo Balb/C mice model. Secretion of pro-inflammatory cytokines and the increase in CD + 4 populations indicate the activation of immune cells in the current study. Immunotherapy by the help of metal nano conjugate can be an effective tool to eradicate the cancer cells from the system.
Identifiants
pubmed: 31923815
pii: S1043-4666(19)30387-4
doi: 10.1016/j.cyto.2019.154958
pii:
doi:
Substances chimiques
Adjuvants, Immunologic
0
Copper
789U1901C5
Chitosan
9012-76-4
cuprous oxide
T8BEA5064F
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
154958Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.