The gastrointestinal pathogen Campylobacter jejuni metabolizes sugars with potential help from commensal Bacteroides vulgatus.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
07 01 2020
07 01 2020
Historique:
received:
18
05
2019
accepted:
04
12
2019
entrez:
12
1
2020
pubmed:
12
1
2020
medline:
5
5
2021
Statut:
epublish
Résumé
Although the gastrointestinal pathogen Campylobacter jejuni was considered asaccharolytic, >50% of sequenced isolates possess an operon for L-fucose utilization. In C. jejuni NCTC11168, this pathway confers L-fucose chemotaxis and competitive colonization advantages in the piglet diarrhea model, but the catabolic steps remain unknown. Here we solved the putative dehydrogenase structure, resembling FabG of Burkholderia multivorans. The C. jejuni enzyme, FucX, reduces L-fucose and D-arabinose in vitro and both sugars are catabolized by fuc-operon encoded enzymes. This enzyme alone confers chemotaxis to both sugars in a non-carbohydrate-utilizing C. jejuni strain. Although C. jejuni lacks fucosidases, the organism exhibits enhanced growth in vitro when co-cultured with Bacteroides vulgatus, suggesting scavenging may occur. Yet, when excess amino acids are available, C. jejuni prefers them to carbohydrates, indicating a metabolic hierarchy exists. Overall this study increases understanding of nutrient metabolism by this pathogen, and identifies interactions with other gut microbes.
Identifiants
pubmed: 31925306
doi: 10.1038/s42003-019-0727-5
pii: 10.1038/s42003-019-0727-5
pmc: PMC6946681
doi:
Substances chimiques
Mucins
0
Sugars
0
Fucose
28RYY2IV3F
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2Subventions
Organisme : CIHR
ID : MOP84224
Pays : Canada
Références
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Environ Microbiol. 2018 Apr;20(4):1374-1388
pubmed: 29318721
J Glycomics Lipidomics. 2012 May 1;Suppl 1:002
pubmed: 24533224
J Bacteriol. 2003 Mar;185(6):2009-16
pubmed: 12618466
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
PLoS Negl Trop Dis. 2013;7(1):e2036
pubmed: 23383356
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
PLoS One. 2016 Jan 22;11(1):e0147438
pubmed: 26800369
J Bacteriol. 2007 Jan;189(2):422-9
pubmed: 17085576
Vox Sang. 1986;51(3):161-71
pubmed: 2433836
J Biol Chem. 2004 May 28;279(22):23431-7
pubmed: 15026423
Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):12901-6
pubmed: 25139987
Nat Chem Biol. 2010 Oct;6(10):724-32
pubmed: 20852610
Analyst. 2011 Nov 21;136(22):4780-6
pubmed: 21955997
J Bacteriol. 1985 Jan;161(1):435-7
pubmed: 3918008
J Biol Chem. 2006 Sep 15;281(37):27378-88
pubmed: 16849334
Front Cell Infect Microbiol. 2014 Sep 29;4:137
pubmed: 25325018
Viruses. 2018 Jun 16;10(6):
pubmed: 29914170
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21
pubmed: 20057044
Pediatr Res. 2014 May;75(5):675-9
pubmed: 24522101
Proc Natl Acad Sci U S A. 1995 Jan 3;92(1):285-9
pubmed: 7816834
Science. 2003 Mar 28;299(5615):2074-6
pubmed: 12663928
Genome Biol. 2014;15(12):550
pubmed: 25516281
Science. 2005 Mar 18;307(5716):1778-81
pubmed: 15774760
Annu Rev Biochem. 1985;54:631-64
pubmed: 3896128
Methods Enzymol. 1997;276:307-26
pubmed: 27754618
Anal Chem. 2004 Feb 1;76(3):619-26
pubmed: 14750855
Nature. 2016 Apr 25;533(7602):255-9
pubmed: 27111508
Microb Genom. 2018 Jan;4(8):
pubmed: 30024366
Infect Immun. 2000 Nov;68(11):6176-81
pubmed: 11035722
mBio. 2017 May 9;8(3):
pubmed: 28487428
Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):7194-9
pubmed: 21482772
BMC Bioinformatics. 2009 May 27;10:161
pubmed: 19473525
ACS Infect Dis. 2018 May 11;4(5):758-770
pubmed: 29411974
Front Microbiol. 2016 Nov 22;7:1877
pubmed: 27920773
Appl Environ Microbiol. 2005 Jun;71(6):3351-4
pubmed: 15933040
J Mol Biol. 2007 Sep 21;372(3):774-97
pubmed: 17681537
Glycobiology. 2017 Jul 1;27(7):601-618
pubmed: 28430973
J Bacteriol. 1971 Apr;106(1):90-6
pubmed: 4928018
Nature. 2012 Dec 6;492(7427):113-7
pubmed: 23160491
Nature. 1992 Jan 30;355(6359):472-5
pubmed: 18481394
Mol Microbiol. 2015 Dec;98(5):809-30
pubmed: 26259566
Appl Environ Microbiol. 2015 Jun;81(11):3880-8
pubmed: 25819967
Nature. 2014 Oct 30;514(7524):638-41
pubmed: 25274297
BMC Genomics. 2015 Jul 04;16:498
pubmed: 26141822
Mol Microbiol. 2016 Aug;101(4):575-89
pubmed: 27145048
ISME J. 2013 Oct;7(10):1933-43
pubmed: 23739052
Front Cell Infect Microbiol. 2012 Feb 07;2:5
pubmed: 22919597
J Bacteriol. 2011 Aug;193(15):4025-6
pubmed: 21622758
Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1002-11
pubmed: 16929101
Mol Microbiol. 2016 Dec;102(5):882-908
pubmed: 27611014
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
mBio. 2014 Mar 11;5(2):e00909-14
pubmed: 24618254
RSC Adv. 2018 Mar 2;8(17):9243-9252
pubmed: 35541856
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
Eur J Microbiol Immunol (Bp). 2012 Mar;2(1):24-31
pubmed: 24611118
Metabolomics. 2018 Oct 23;14(11):144
pubmed: 30830405
Structure. 2011 Nov 9;19(11):1603-14
pubmed: 22078560
J Bacteriol. 2011 Mar;193(5):1065-75
pubmed: 21193610
Mutat Res. 1991 Nov;255(3):265-71
pubmed: 1719398
Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9833-8
pubmed: 10449780
FEBS Lett. 2018 Dec;592(23):3865-3897
pubmed: 29608212
Nat Rev Microbiol. 2010 Nov;8(11):765-78
pubmed: 20948550
Food Funct. 2018 Apr 25;9(4):2300-2312
pubmed: 29561006
Biochemistry. 2006 Dec 12;45(49):14582-97
pubmed: 17144652
Glycobiology. 2016 Aug;26(8):871-879
pubmed: 26941394
Nature. 2013 Oct 3;502(7469):96-9
pubmed: 23995682
PLoS One. 2016 May 26;11(5):e0155405
pubmed: 27228122
Biochemistry. 2013 Jan 8;52(1):239-53
pubmed: 23214453