Human iPSC-derived microglia: A growing toolset to study the brain's innate immune cells.
cerebral organoids
chimeric
induced pluripotent stem cells
microglia
microglia-like cells
xenotransplantation
Journal
Glia
ISSN: 1098-1136
Titre abrégé: Glia
Pays: United States
ID NLM: 8806785
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
25
06
2019
revised:
21
12
2019
accepted:
30
12
2019
pubmed:
12
1
2020
medline:
3
2
2021
entrez:
12
1
2020
Statut:
ppublish
Résumé
Recent advances in the generation of microglia from human induced pluripotent stem cells (iPSCs) have provided exciting new approaches to examine and decipher the biology of microglia. As these techniques continue to evolve to encompass more complex in situ and in vivo paradigms, so too have they begun to yield novel scientific insight into the genetics and function of human microglia. As such, researchers now have access to a toolset comprised of three unique "flavors" of iPSC-derived microglia: in vitro microglia (iMGs), organoid microglia (oMGs), and xenotransplanted microglia (xMGs). The goal of this review is to discuss the variety of research applications that each of these techniques enables and to highlight recent discoveries that these methods have begun to uncover. By presenting the research paradigms in which each model has been successful, as well as the key benefits and limitations of each approach, it is our hope that this review will help interested researchers to incorporate these techniques into their studies, collectively advancing our understanding of human microglia biology.
Identifiants
pubmed: 31926038
doi: 10.1002/glia.23781
pmc: PMC7813153
mid: NIHMS1660410
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
721-739Subventions
Organisme : Foundation for the National Institutes of Health
ID : AG048099
Pays : International
Organisme : NIA NIH HHS
ID : RF1 AG048099
Pays : United States
Organisme : NIGMS NIH HHS
ID : DP2 GM119164
Pays : United States
Organisme : NIA NIH HHS
ID : P30 AG066519
Pays : United States
Organisme : NIA NIH HHS
ID : P50 AG016573
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG055524
Pays : United States
Organisme : Foundation for the National Institutes of Health
ID : AG016573
Pays : International
Organisme : NIDA NIH HHS
ID : RF1 DA048813
Pays : United States
Organisme : Foundation for the National Institutes of Health
ID : AG00096
Pays : International
Organisme : Foundation for the National Institutes of Health
ID : AG055524
Pays : International
Organisme : NIA NIH HHS
ID : R01 AG056303
Pays : United States
Organisme : NIA NIH HHS
ID : T32 AG000096
Pays : United States
Organisme : Foundation for the National Institutes of Health
ID : AG056303
Pays : International
Informations de copyright
© 2020 Wiley Periodicals, Inc.
Références
N Engl J Med. 2016 Apr 21;374(16):1552-63
pubmed: 27028561
N Engl J Med. 2013 Jan 10;368(2):107-16
pubmed: 23150908
Immunity. 2017 Jul 18;47(1):183-198.e6
pubmed: 28723550
Stem Cells. 2019 Jun;37(6):724-730
pubmed: 30801863
J Neurochem. 2018 Dec;147(6):784-802
pubmed: 30152135
Proc Natl Acad Sci U S A. 2015 Dec 22;112(51):15672-7
pubmed: 26644564
Neuron. 2017 May 17;94(4):759-773.e8
pubmed: 28521131
J Neuroimmune Pharmacol. 2019 Mar;14(1):110-119
pubmed: 30194646
Acta Neuropathol. 2016 Mar;131(3):347-63
pubmed: 26711460
Trends Neurosci. 2014 Mar;37(3):125-35
pubmed: 24388427
Nat Genet. 2013 Dec;45(12):1452-8
pubmed: 24162737
Mol Neurodegener. 2019 Mar 5;14(1):12
pubmed: 30832693
Nat Neurosci. 2018 Oct;21(10):1370-1379
pubmed: 30250265
Nat Methods. 2015 Jul;12(7):671-8
pubmed: 26005811
Front Cell Neurosci. 2013 Apr 23;7:44
pubmed: 23630462
ASN Neuro. 2018 Jan-Dec;10:1759091418781921
pubmed: 29932344
PLoS One. 2011;6(10):e26317
pubmed: 22046273
Cell. 2017 Jun 15;169(7):1276-1290.e17
pubmed: 28602351
Dev Biol. 2016 Dec 15;420(2):199-209
pubmed: 27402594
Nat Neurosci. 2017 May;20(5):753-759
pubmed: 28253233
Nat Biotechnol. 2014 Apr;32(4):364-72
pubmed: 24633240
Neuron. 2018 Jun 27;98(6):1141-1154.e7
pubmed: 29861287
JAMA. 1997 Oct 22-29;278(16):1349-56
pubmed: 9343467
Mol Autism. 2017 Mar 20;8:11
pubmed: 28321286
Science. 2000 Dec 1;290(5497):1768-71
pubmed: 11099416
Nat Genet. 2009 Oct;41(10):1088-93
pubmed: 19734902
Neuron. 2018 Nov 21;100(4):783-797
pubmed: 30465765
Nature. 2017 Jan 26;541(7638):481-487
pubmed: 28099414
J Neurol Neurosurg Psychiatry. 2017 Mar;88(3):266-271
pubmed: 27799296
Cell. 2015 Mar 12;160(6):1061-71
pubmed: 25728668
Annu Rev Physiol. 2017 Feb 10;79:619-643
pubmed: 27959620
Sci Rep. 2017 Aug 21;7(1):9003
pubmed: 28827786
J Neuroimmunol. 2005 Mar;160(1-2):210-8
pubmed: 15710475
J Neurovirol. 2019 Jun;25(3):342-353
pubmed: 30767174
Neurobiol Aging. 2013 Dec;34(12):2890.e1-5
pubmed: 23870839
Clin Immunol. 2018 Apr;189:63-74
pubmed: 27094466
Nat Rev Neurosci. 2018 Aug;19(8):445-452
pubmed: 29977068
Neurobiol Dis. 2011 Jan;41(1):96-103
pubmed: 20816784
Science. 2012 Apr 6;336(6077):86-90
pubmed: 22442384
Neuron. 2019 Mar 20;101(6):1099-1108.e6
pubmed: 30737131
Neurotox Res. 2009 Oct;16(3):238-54
pubmed: 19526281
Stem Cell Reports. 2017 Jun 6;8(6):1516-1524
pubmed: 28528700
Neuron. 2014 Apr 16;82(2):380-97
pubmed: 24742461
Glia. 2016 Nov;64(11):1801-40
pubmed: 27634048
AIDS Res Hum Retroviruses. 2019 Jul;35(7):634-641
pubmed: 30880401
Sci Adv. 2017 Dec 06;3(12):e1701211
pubmed: 29226242
Immunity. 2017 Sep 19;47(3):566-581.e9
pubmed: 28930663
Mol Neurodegener. 2018 Dec 22;13(1):67
pubmed: 30577865
Neuron. 2016 May 18;90(4):724-39
pubmed: 27196974
Nat Genet. 2017 Sep;49(9):1373-1384
pubmed: 28714976
Nat Neurosci. 2019 Dec;22(12):2111-2116
pubmed: 31659342
Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20284-9
pubmed: 24277810
Neuron. 2017 Apr 19;94(2):278-293.e9
pubmed: 28426964
Mol Neurodegener. 2017 May 26;12(1):43
pubmed: 28549481
Nature. 2013 Sep 19;501(7467):373-9
pubmed: 23995685
eNeuro. 2018 Nov 20;5(6):
pubmed: 30460331
J Immunol. 2002 May 1;168(9):4747-55
pubmed: 11971025
Nat Neurosci. 2014 Jan;17(1):131-43
pubmed: 24316888
J Cell Biol. 2018 Feb 5;217(2):459-472
pubmed: 29196460
Neuron. 2017 Dec 6;96(5):1041-1054.e5
pubmed: 29103808
Nat Neurosci. 2006 Jul;9(7):917-24
pubmed: 16732273
Science. 2010 Nov 5;330(6005):841-5
pubmed: 20966214
J Exp Med. 2015 Mar 9;212(3):287-95
pubmed: 25732305
Front Neuroanat. 2015 Apr 14;9:45
pubmed: 25926775
Cell. 2015 Jul 16;162(2):375-390
pubmed: 26186191
Oncotarget. 2017 Oct 4;8(54):93219-93226
pubmed: 29190991
Nat Commun. 2019 Jul 19;10(1):3215
pubmed: 31324781
Cell Rep. 2018 Jan 16;22(3):832-847
pubmed: 29346778
Immunobiology. 2016 Oct;221(10):1080-90
pubmed: 27371974
Front Cell Neurosci. 2018 Aug 06;12:242
pubmed: 30127723
Acta Neuropathol. 2019 Aug;138(2):251-273
pubmed: 31006066
Science. 2017 Jun 23;356(6344):
pubmed: 28546318
Biochim Biophys Acta. 2016 Oct;1862(10):1847-60
pubmed: 27425031
Nat Protoc. 2010 Sep;5(9):1481-94
pubmed: 20725065
Mol Neurodegener. 2014 Jun 03;9:20
pubmed: 24893973
Proc Natl Acad Sci U S A. 2019 Dec 10;116(50):25293-25303
pubmed: 31772018
Nat Commun. 2018 Mar 26;9(1):1228
pubmed: 29581545
Nature. 2017 May 4;545(7652):48-53
pubmed: 28445462
NPJ Parkinsons Dis. 2019 Apr 5;5:5
pubmed: 30963107
Alzheimers Dement. 2019 Mar;15(3):453-464
pubmed: 30442540
Nature. 2019 Jun;570(7762):523-527
pubmed: 31168097
PLoS One. 2016 Sep 13;11(9):e0161969
pubmed: 27622770
N Engl J Med. 2013 Jan 10;368(2):117-27
pubmed: 23150934
Cell Rep. 2018 Aug 28;24(9):2300-2311
pubmed: 30157425
Blood. 2011 Sep 15;118(11):3119-28
pubmed: 21791433
Eur J Pharmacol. 2015 Jul 15;759:101-17
pubmed: 25814260
Neurobiol Aging. 2017 Jul;55:115-122
pubmed: 28434692
Neuron. 2019 Sep 25;103(6):1016-1033.e10
pubmed: 31375314
Nat Neurosci. 2017 Aug;20(8):1052-1061
pubmed: 28628103
Neuron. 2018 Jun 27;98(6):1170-1183.e8
pubmed: 29861285
Nat Immunol. 2012 Jun 24;13(8):753-60
pubmed: 22729249
Cold Spring Harb Perspect Med. 2012 Dec 01;2(12):a007443
pubmed: 23143844
Nat Genet. 2019 Mar;51(3):414-430
pubmed: 30820047
Open Biol. 2013 Dec 18;3(12):130181
pubmed: 24352739
Nat Med. 2016 Nov;22(11):1358-1367
pubmed: 27668937
Nat Neurosci. 2005 Jun;8(6):752-8
pubmed: 15895084
Front Neurol. 2015 Apr 08;6:81
pubmed: 25904895
J Clin Invest. 1987 Jun;79(6):1549-57
pubmed: 3034976
Nat Genet. 2019 Mar;51(3):404-413
pubmed: 30617256
Nat Commun. 2018 Oct 9;9(1):4167
pubmed: 30301888
Nat Neurosci. 2013 Mar;16(3):273-80
pubmed: 23334579
Stem Cell Reports. 2018 Apr 10;10(4):1294-1307
pubmed: 29606617
Nat Med. 2019 Jun;25(6):988-1000
pubmed: 31086348
Mol Neurodegener. 2018 Sep 6;13(1):49
pubmed: 30185230
Nat Genet. 2009 Oct;41(10):1094-9
pubmed: 19734903
J Neurosci. 2016 Feb 24;36(8):2383-90
pubmed: 26911687
Front Cell Neurosci. 2013 Jan 30;7:3
pubmed: 23386810
Science. 2005 May 27;308(5726):1314-8
pubmed: 15831717
Hum Mol Genet. 2018 Jan 1;27(1):41-52
pubmed: 29048558
Nat Biotechnol. 2018 Jun;36(5):432-441
pubmed: 29658944
Acta Neuropathol. 2016 Jun;131(6):925-33
pubmed: 26754641
Neuron. 2012 May 24;74(4):691-705
pubmed: 22632727
Proc Natl Acad Sci U S A. 2018 Jul 3;115(27):7117-7122
pubmed: 29915057
Stem Cell Reports. 2017 Jun 6;8(6):1727-1742
pubmed: 28591653
Semin Cell Dev Biol. 2019 Oct;94:138-151
pubmed: 31112798
Exp Neurol. 2012 Apr;234(2):340-50
pubmed: 22119425
Nat Neurosci. 2017 Aug;20(8):1162-1171
pubmed: 28671693