Enhanced neuronal differentiation of neural stem cells with mechanically enhanced touch-spun nanofibrous scaffolds.

Animals Cell Differentiation / physiology Humans Nanofibers / chemistry Neural Stem Cells / cytology Polyesters / chemistry Polymers / chemistry Tissue Engineering / methods Tissue Scaffolds / chemistry
Electrospinning Nanofiber Neural stem cells Tissue engineering Touch-spinning

Journal

Nanomedicine : nanotechnology, biology, and medicine
ISSN: 1549-9642
Titre abrégé: Nanomedicine
Pays: United States
ID NLM: 101233142

Informations de publication

Date de publication:
02 2020
Historique:
received: 06 06 2019
revised: 23 12 2019
accepted: 27 12 2019
pubmed: 14 1 2020
medline: 24 11 2020
entrez: 14 1 2020
Statut: ppublish

Résumé

We studied NE-4C neural cells differentiation on 2D polycaprolactone (PCL) nanofibrous scaffolds with systematically varied mechanical characteristics of nanofibers while retaining an unchanged fiber alignment, diameter, and chemical composition. Our experiments demonstrated that the nanofibers with enhanced mechanical properties are beneficial for the preferential development of neuronal cells vs. glial cells. Electrospun (ES) and touch-spun (TS) nanofibers were fabricated with Young's modulus in the range of 10 MPa to 230 MPa and a fraction of crystallinity from 30% to 80%. The TS fibers undergo a greater drawing ratio and thus approach a greater polymer chain stretching and alignment that resulted in an increased crystallinity. The TS scaffolds demonstrated improved stability in the aqueous cell culture environment, resisting misalignment and entanglement after a period of 2 weeks of swelling followed by 14 days of neural differentiation. The results confirmed that the neurites on the TS fibers have a preferred orientation even after swelling.

Identifiants

DOI: 10.1016/j.nano.2020.102152 PMID: 31927134
pubmed: 31927134
pii: S1549-9634(20)30004-6
doi: 10.1016/j.nano.2020.102152
pii:
doi:

Substances chimiques

Polyesters 0
Polymers 0
polycaprolactone 24980-41-4

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

102152

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Auteurs

Darya Asheghali (D)

Nanostructured Materials Laboratory, The University of Georgia, Athens, GA, USA; Department of Chemistry, The University of Georgia, Athens, GA, USA.

Se-Jun Lee (SJ)

Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC, USA.

Andreas Furchner (A)

Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Berlin, Germany.

Alexey Gruzd (A)

Nanostructured Materials Laboratory, The University of Georgia, Athens, GA, USA; Graduate Program of Polymer, Fibers, and Textile Sciences, USA.

Steven Larson (S)

Department of Physics and Astronomy, The University of Georgia, Athens, GA, USA.

Alexander Tokarev (A)

Nanostructured Materials Laboratory, The University of Georgia, Athens, GA, USA.

Seth Stake (S)

Department of Medicine, The George Washington University, Washington, DC, USA.

Xuan Zhou (X)

Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC, USA.

Karsten Hinrichs (K)

Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Berlin, Germany.

Lijie G Zhang (LG)

Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC, USA; Department of Medicine, The George Washington University, Washington, DC, USA; Department of Biomedical Engineering, The George Washington University, Washington, DC, USA. Electronic address: lgzhang@email.gwu.edu.

Sergiy Minko (S)

Nanostructured Materials Laboratory, The University of Georgia, Athens, GA, USA; Department of Chemistry, The University of Georgia, Athens, GA, USA; Graduate Program of Polymer, Fibers, and Textile Sciences, USA. Electronic address: sminko@uga.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Enhanced neuronal differentiation of neural...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Enhanced neuronal differentiation of neural...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Enhanced neuronal differentiation of neural...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanofibres › Enhanced neuronal differentiation of neural...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches neurales › Enhanced neuronal differentiation of neural...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Polyesters › Enhanced neuronal differentiation of neural...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Enhanced neuronal differentiation of neural...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Ingénierie tissulaire › Enhanced neuronal differentiation of neural...
questionsmedicales.fr › Équipement et fournitures › Prothèses et implants › Structures d'échafaudage tissulaires › Enhanced neuronal differentiation of neural...