Yeast-encapsulated essential oils: a new perspective as an environmentally friendly larvicide.
Encapsulation
Essential oil
Larvicide
Saccharomyces cerevisiae
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
13 Jan 2020
13 Jan 2020
Historique:
received:
20
06
2019
accepted:
29
12
2019
entrez:
15
1
2020
pubmed:
15
1
2020
medline:
15
5
2020
Statut:
epublish
Résumé
Effective mosquito control approaches incorporate both adult and larval stages. For the latter, physical, biological, and chemical control have been used with varying results. Successful control of larvae has been demonstrated using larvicides including insect growth regulators, e.g. the organophosphate temephos, as well as various entomopathogenic microbial species. However, a variety of health and environmental issues are associated with some of these. Laboratory trials of essential oils (EO) have established the larvicidal activity of these substances, but there are currently no commercially available EO-based larvicides. Here we report on the development of a new approach to mosquito larval control using a novel, yeast-based delivery system for EO. Food-grade orange oil (OO) was encapsulated into yeast cells following an established protocol. To prevent environmental contamination, a proprietary washing strategy was developed to remove excess EO that is adsorbed to the cell exterior during the encapsulation process. The OO-loaded yeast particles were then characterized for OO loading, and tested for efficacy against Aedes aegypti larvae. The composition of encapsulated OO extracted from the yeast microparticles was demonstrated not to differ from that of un-encapsulated EO when analyzed by high performance liquid chromatography. After lyophilization, the oil in the larvicide comprised 26-30 percentage weight (wt%), and is consistent with the 60-65% reduction in weight observed after the drying process. Quantitative bioassays carried with Liverpool and Rockefeller Ae. aegypti strains in three different laboratories presented LD The larvicide based on OO encapsulated in yeast was shown to be highly active (LD
Sections du résumé
BACKGROUND
BACKGROUND
Effective mosquito control approaches incorporate both adult and larval stages. For the latter, physical, biological, and chemical control have been used with varying results. Successful control of larvae has been demonstrated using larvicides including insect growth regulators, e.g. the organophosphate temephos, as well as various entomopathogenic microbial species. However, a variety of health and environmental issues are associated with some of these. Laboratory trials of essential oils (EO) have established the larvicidal activity of these substances, but there are currently no commercially available EO-based larvicides. Here we report on the development of a new approach to mosquito larval control using a novel, yeast-based delivery system for EO.
METHODS
METHODS
Food-grade orange oil (OO) was encapsulated into yeast cells following an established protocol. To prevent environmental contamination, a proprietary washing strategy was developed to remove excess EO that is adsorbed to the cell exterior during the encapsulation process. The OO-loaded yeast particles were then characterized for OO loading, and tested for efficacy against Aedes aegypti larvae.
RESULTS
RESULTS
The composition of encapsulated OO extracted from the yeast microparticles was demonstrated not to differ from that of un-encapsulated EO when analyzed by high performance liquid chromatography. After lyophilization, the oil in the larvicide comprised 26-30 percentage weight (wt%), and is consistent with the 60-65% reduction in weight observed after the drying process. Quantitative bioassays carried with Liverpool and Rockefeller Ae. aegypti strains in three different laboratories presented LD
CONCLUSIONS
CONCLUSIONS
The larvicide based on OO encapsulated in yeast was shown to be highly active (LD
Identifiants
pubmed: 31931883
doi: 10.1186/s13071-019-3870-4
pii: 10.1186/s13071-019-3870-4
pmc: PMC6958686
doi:
Substances chimiques
Insecticides
0
Oils, Volatile
0
Plant Oils
0
orange oil
AKN3KSD11B
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
19Subventions
Organisme : CDC HHS
ID : 200-2017-93140
Pays : United States
Références
Neotrop Entomol. 2017 Jun;46(3):243-255
pubmed: 28401481
Parasitol Res. 2016 Apr;115(4):1691-703
pubmed: 26796022
J Agric Food Chem. 2019 May 1;67(17):4746-4753
pubmed: 30966749
Bioresour Technol. 2006 Dec;97(18):2481-4
pubmed: 16815011
PLoS Negl Trop Dis. 2015 Jan 15;9(1):e0003406
pubmed: 25590626
PLoS Negl Trop Dis. 2017 Jul 20;11(7):e0005625
pubmed: 28727779
Mem Inst Oswaldo Cruz. 2016 Jul 4;111(7):443-9
pubmed: 27384083
BMC Dev Biol. 2008 Sep 13;8:82
pubmed: 18789161
Antiviral Res. 2010 Feb;85(2):328-45
pubmed: 19857523
Front Physiol. 2019 Apr 09;10:152
pubmed: 31024326
Mem Inst Oswaldo Cruz. 2003 Jun;98(4):569-71
pubmed: 12937776
PLoS One. 2016 Mar 23;11(3):e0151403
pubmed: 27007411
Insect Biochem Mol Biol. 2004 Jul;34(7):653-65
pubmed: 15242706
Trends Plant Sci. 2016 Dec;21(12):1000-1007
pubmed: 27789158
J Insect Sci. 2004;4:19
pubmed: 15861235
Southeast Asian J Trop Med Public Health. 2005 Nov;36(6):1412-22
pubmed: 16610643
Food Chem Toxicol. 2008 Feb;46(2):446-75
pubmed: 17996351
Mem Inst Oswaldo Cruz. 2004 Aug;99(5):541-4
pubmed: 15543421
Parasitol Res. 2014 Jul;113(7):2647-54
pubmed: 24781026
Trop Biomed. 2006 Dec;23(2):180-5
pubmed: 17322820
J Agric Food Chem. 2004 Jul 14;52(14):4395-400
pubmed: 15237942
Parasitol Res. 2012 Jul;111(1):173-8
pubmed: 22231268
Parasitol Res. 2012 Oct;111(4):1757-69
pubmed: 22797605
Asian Pac J Trop Biomed. 2012 Feb;2(2):152-5
pubmed: 23569887
J Econ Entomol. 2016 Feb;109(1):360-5
pubmed: 26357845
Bioresour Technol. 2003 Aug;89(1):99-102
pubmed: 12676507
Parasitol Res. 2014 Feb;113(2):565-92
pubmed: 24265058
Malar J. 2019 Feb 26;18(1):55
pubmed: 30808348
Pestic Biochem Physiol. 2018 Oct;151:73-75
pubmed: 30704716
J Colloid Interface Sci. 2015 Oct 15;456:190-6
pubmed: 26125515
PLoS Negl Trop Dis. 2017 Jul 27;11(7):e0005632
pubmed: 28749942
J Microencapsul. 1998 Nov-Dec;15(6):761-73
pubmed: 9818954
Parasit Vectors. 2014 Sep 04;7:426
pubmed: 25190294
Comp Biochem Physiol C Toxicol Pharmacol. 2001 Nov;130(3):325-37
pubmed: 11701389
J Am Mosq Control Assoc. 1994 Sep;10(3):344-7
pubmed: 7807075
J Am Mosq Control Assoc. 1991 Jun;7(2):210-37
pubmed: 1680152