MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells.
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
12
08
2019
accepted:
15
11
2019
pubmed:
15
1
2020
medline:
6
5
2020
entrez:
15
1
2020
Statut:
ppublish
Résumé
The ultimate goal of biological super-resolution fluorescence microscopy is to provide three-dimensional resolution at the size scale of a fluorescent marker. Here we show that by localizing individual switchable fluorophores with a probing donut-shaped excitation beam, MINFLUX nanoscopy can provide resolutions in the range of 1 to 3 nm for structures in fixed and living cells. This progress has been facilitated by approaching each fluorophore iteratively with the probing-donut minimum, making the resolution essentially uniform and isotropic over scalable fields of view. MINFLUX imaging of nuclear pore complexes of a mammalian cell shows that this true nanometer-scale resolution is obtained in three dimensions and in two color channels. Relying on fewer detected photons than standard camera-based localization, MINFLUX nanoscopy is poised to open a new chapter in the imaging of protein complexes and distributions in fixed and living cells.
Identifiants
pubmed: 31932776
doi: 10.1038/s41592-019-0688-0
pii: 10.1038/s41592-019-0688-0
doi:
Substances chimiques
Fluorescent Dyes
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
217-224Commentaires et corrections
Type : CommentIn
Type : CommentIn
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