Comparative analysis of primary metabolites and transcriptome changes between ungrafted and pumpkin-grafted watermelon during fruit development.
Amino acid
Citrullus lanatus
Grafting
Metabolism
Organic acids
Sugar
Transcriptome
qRT-PCR
Journal
PeerJ
ISSN: 2167-8359
Titre abrégé: PeerJ
Pays: United States
ID NLM: 101603425
Informations de publication
Date de publication:
2020
2020
Historique:
received:
26
06
2019
accepted:
20
11
2019
entrez:
15
1
2020
pubmed:
15
1
2020
medline:
15
1
2020
Statut:
epublish
Résumé
Grafting has been reported as a factor that influences fruit quality. However, a comprehensive study of the metabolic profile related to fruit quality and the underlying molecular mechanism in grafted watermelon has not been carried out. Metabolomics and transcriptome analysis were performed on both pumpkin-grafted watermelon and ungrafted watermelon at different developmental stages. In total, 56 primary metabolites were identified with either high or low abundance between ungrafted and pumpkin-grafted watermelon. The results indicated that ornithine, arginine, lysine (amino acids), glucose, sucrose, glucosamine (sugars), malic acid, fumaric acid and succinic acid (organic acids) were among the dominant metabolites influencing fruit quality. Additionally, comparative RNA sequence analysis on grafted and ungrafted watermelon yielded 729, 174, 128 and 356 differentially expressed genes at 10, 18, 26 and 34 days after pollination (DAP), respectively. Functional annotations of these genes indicated that grafting significantly altered the biological and metabolic processes related to fruit quality. Our comparative metabolomics and transcriptome analysis revealed that
Identifiants
pubmed: 31934503
doi: 10.7717/peerj.8259
pii: 8259
pmc: PMC6951286
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e8259Informations de copyright
© 2020 Aslam et al.
Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.
Références
BMC Genomics. 2017 Jan 3;18(1):3
pubmed: 28049426
Metabolites. 2019 Mar 22;9(3):null
pubmed: 30909447
Curr Opin Plant Biol. 2012 Jun;15(3):315-21
pubmed: 22366488
Food Chem. 2011 Aug 1;127(3):926-33
pubmed: 25214080
Front Plant Sci. 2018 Nov 26;9:1716
pubmed: 30542359
Plant Cell. 2012 Jun;24(6):2328-51
pubmed: 22751214
Mol Plant Microbe Interact. 2012 Jul;25(7):976-92
pubmed: 22414438
PLoS One. 2018 Jan 11;13(1):e0190096
pubmed: 29324867
J Exp Bot. 1996 Aug;47 Spec No:1179-85
pubmed: 21245246
Nutrition. 2007 Mar;23(3):261-6
pubmed: 17352962
Front Plant Sci. 2017 Mar 06;8:291
pubmed: 28321230
Int J Mol Sci. 2018 Dec 07;19(12):null
pubmed: 30544504
Plant Mol Biol. 1994 Feb;24(3):417-27
pubmed: 8123785
Plant Physiol. 2004 Sep;136(1):2483-99
pubmed: 15375205
Plant Cell Physiol. 2015 Jun;56(6):1084-96
pubmed: 25713174
Plant Cell Environ. 2010 Oct;33(10):1656-70
pubmed: 20492555
PLoS One. 2016 Jun 09;11(6):e0157022
pubmed: 27280887
Mol Genet Genomics. 2012 Aug;287(8):663-78
pubmed: 22806345
OMICS. 2012 May;16(5):284-7
pubmed: 22455463
Front Plant Sci. 2017 Jun 14;8:1030
pubmed: 28659962
Genome Biol. 2014;15(12):550
pubmed: 25516281
Microb Cell Fact. 2007 May 24;6:16
pubmed: 17521452
J Agric Food Chem. 2006 Apr 5;54(7):2593-7
pubmed: 16569049
Plant Physiol Biochem. 2018 Sep;130:289-302
pubmed: 30036858
Nucleic Acids Res. 2010 Apr;38(6):1767-71
pubmed: 20015970
Bioinformatics. 2018 Sep 1;34(17):i884-i890
pubmed: 30423086
Nat Genet. 2000 May;25(1):25-9
pubmed: 10802651
J Plant Physiol. 2010 May 15;167(8):589-96
pubmed: 20036442
Plant Cell. 2017 Sep;29(9):2249-2268
pubmed: 28814642
BMC Genomics. 2011 Sep 21;12:454
pubmed: 21936920
PLoS One. 2015 Jun 25;10(6):e0130865
pubmed: 26110539
Nat Biotechnol. 2015 Mar;33(3):290-5
pubmed: 25690850
Annu Rev Biochem. 1978;47:532-606
pubmed: 354503
Physiol Plant. 2002 Feb;114(2):259-270
pubmed: 11903973
Int J Mol Sci. 2019 Jul 23;20(14):null
pubmed: 31340498
Sci Rep. 2017 Apr 05;7:45933
pubmed: 28378825
Mol Genet Genomics. 2016 Apr;291(2):621-33
pubmed: 26500104
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
J Sci Food Agric. 2017 Mar;97(5):1646-1655
pubmed: 27436661
Nat Methods. 2015 Apr;12(4):357-60
pubmed: 25751142
Nucleic Acids Res. 2008 Jan;36(Database issue):D480-4
pubmed: 18077471
Amino Acids. 2001;20(3):301-17
pubmed: 11354606
Proc Natl Acad Sci U S A. 2005 Sep 20;102(38):13681-6
pubmed: 16157883
Plant Physiol. 1997 Sep;115(1):273-82
pubmed: 9306701
PLoS One. 2015 Jun 16;10(6):e0130267
pubmed: 26079257
Plant Cell. 1999 Feb;11(2):177-89
pubmed: 9927637
Front Plant Sci. 2013 Mar 12;4:44
pubmed: 23487525
Plant Physiol. 1989 Dec;91(4):1527-34
pubmed: 16667212
Plant Physiol. 1992 Jun;99(2):434-8
pubmed: 16668903
Plant Cell Physiol. 2015 Feb;56(2):311-21
pubmed: 25527829