HYPERsol: High-Quality Data from Archival FFPE Tissue for Clinical Proteomics.

Formaldehyde Paraffin Embedding Proteomics Reproducibility of Results Tissue Fixation
FFPE S-Trap clinical proteomics immunohistochemistry sample preparation tissue

Journal

Journal of proteome research
ISSN: 1535-3907
Titre abrégé: J Proteome Res
Pays: United States
ID NLM: 101128775

Informations de publication

Date de publication:
07 02 2020
Historique:
pubmed: 15 1 2020
medline: 22 6 2021
entrez: 15 1 2020
Statut: ppublish

Résumé

Massive formalin-fixed, paraffin-embedded (FFPE) tissue archives exist worldwide, representing an invaluable resource for clinical proteomics research. However, current protocols for FFPE proteomics lack standardization, efficiency, reproducibility, and scalability. Here we present high-yield protein extraction and recovery by direct solubilization (HYPERsol), an optimized workflow using ultrasonication and S-Trap sample processing that enables proteome coverage and quantification from FFPE samples comparable to that achieved from flash-frozen tissue (average

Identifiants

DOI: 10.1021/acs.jproteome.9b00686 PMID: 31935107 PMC: PMC7515636
pubmed: 31935107
doi: 10.1021/acs.jproteome.9b00686
pmc: PMC7515636
mid: NIHMS1620351
doi:

Substances chimiques

Formaldehyde 1HG84L3525

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

973-983

Subventions

Organisme : NCI NIH HHS
ID : P01 CA196539
Pays : United States
Organisme : NCI NIH HHS
ID : K12 CA076931
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA045508
Pays : United States
Organisme : NCATS NIH HHS
ID : TL1 TR001880
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008275
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI118891
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM110174
Pays : United States

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Auteurs

Dylan M Marchione (DM)

Epigenetics Institute, Department of Biochemistry & Biophysics , University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States.
ORCID: 0000-0001-6941-0604

Ilyana Ilieva (I)

Department of Pathology and Laboratory Medicine , University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States.

Kyle Devins (K)

Department of Pathology and Laboratory Medicine , University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States.

Danielle Sharpe (D)

Department of Pathology and Laboratory Medicine , University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States.

Darryl J Pappin (DJ)

Cold Spring Harbor Laboratory , Cold Spring Harbor , New York 11724 , United States.
ProtiFi, LLC , Huntington , New York 11743 , United States.

Benjamin A Garcia (BA)

Epigenetics Institute, Department of Biochemistry & Biophysics , University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States.
ORCID: 0000-0002-2306-1207

John P Wilson (JP)

ProtiFi, LLC , Huntington , New York 11743 , United States.

John B Wojcik (JB)

Department of Pathology and Laboratory Medicine , University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States.
ORCID: 0000-0001-5403-3631

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Classifications MeSH

questionsmedicales.fr › Composés chimiques organiques › Aldéhydes › Formaldéhyde › HYPERsol: High-Quality Data from Archival FFPE...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Inclusion de tissu › Inclusion en paraffine › HYPERsol: High-Quality Data from Archival FFPE...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Protéomique › HYPERsol: High-Quality Data from Archival FFPE...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › HYPERsol: High-Quality Data from Archival FFPE...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Fixation tissulaire › HYPERsol: High-Quality Data from Archival FFPE...