Increased Cardiovascular Risk Associated with Chemical Sensitivity to Perfluoro-Octanoic Acid: Role of Impaired Platelet Aggregation.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
08 01 2020
Historique:
received: 27 11 2019
revised: 20 12 2019
accepted: 03 01 2020
entrez: 16 1 2020
pubmed: 16 1 2020
medline: 2 10 2020
Statut: epublish

Résumé

Perfluoro-alkyl substances (PFAS), particularly perfluoro-octanoic acid (PFOA), are persisting environmental chemicals showing bioaccumulation in human tissues. Recently, exposure to PFAS has been associated with increased prevalence of cardiovascular diseases (CVDs). However, a causal role of PFAS in atherosclerosis pathogenesis is under-investigated. Here, we investigated the effect of PFOA exposure on platelets' function, a key player in atherosclerosis process. PFOA accumulation in platelets was evaluated by liquid chromatography-mass spectrometry. Changes in platelets' membrane fluidity and activation after dose-dependent exposure to PFOA were evaluated by merocyanine 540 (MC540) and anti P-Selectin immune staining at flow cytometry, respectively. Intracellular calcium trafficking was analyzed with Fluo4M probe, time-lapse live imaging. Platelets' aggregation state was also evaluated with Multiplate

Identifiants

pubmed: 31936344
pii: ijms21020399
doi: 10.3390/ijms21020399
pmc: PMC7014465
pii:
doi:

Substances chimiques

Caprylates 0
Fluorocarbons 0
Water Pollutants, Chemical 0
perfluorooctane 6P60ZBK0QL
octanoic acid OBL58JN025

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Déclaration de conflit d'intérêts

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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Auteurs

Luca De Toni (L)

Department of Medicine, Unit of Andrology and Reproductive Medicine, University of Padova, 35128 Padova, Italy.

Claudia Maria Radu (CM)

Department of Medicine, Thrombotic and Haemorrhagic Diseases Unit, Padova University Hospital, 35128 Padova, Italy.
Department of Women's and Children's Health, University of Padova, 35128 Padova, Italy.

Iva Sabovic (I)

Department of Medicine, Unit of Andrology and Reproductive Medicine, University of Padova, 35128 Padova, Italy.

Andrea Di Nisio (A)

Department of Medicine, Unit of Andrology and Reproductive Medicine, University of Padova, 35128 Padova, Italy.

Stefano Dall'Acqua (S)

Department of Pharmacological Sciences, University of Padova, 35128 Padova, Italy.

Diego Guidolin (D)

Department of Neuroscience, Section of Anatomy, University of Padova, 35128 Padova, Italy.

Salvatore Spampinato (S)

Fondazione Foresta ONLUS, 35128 Padova, Italy.

Elena Campello (E)

Department of Medicine, Thrombotic and Haemorrhagic Diseases Unit, Padova University Hospital, 35128 Padova, Italy.

Paolo Simioni (P)

Department of Medicine, Thrombotic and Haemorrhagic Diseases Unit, Padova University Hospital, 35128 Padova, Italy.

Carlo Foresta (C)

Department of Medicine, Unit of Andrology and Reproductive Medicine, University of Padova, 35128 Padova, Italy.

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Classifications MeSH