Laminin is the ECM niche for trophoblast stem cells.
Animals
Blastocyst
/ cytology
Cell Adhesion
/ genetics
Cell Proliferation
/ genetics
Cells, Cultured
Collagen
/ metabolism
Extracellular Matrix
/ metabolism
Female
Gene Knock-In Techniques
Integrins
/ metabolism
Laminin
/ genetics
Mice
Mice, Transgenic
Pregnancy
Stem Cell Niche
/ genetics
Stem Cells
/ metabolism
Trophoblasts
/ metabolism
Journal
Life science alliance
ISSN: 2575-1077
Titre abrégé: Life Sci Alliance
Pays: United States
ID NLM: 101728869
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
05
08
2019
revised:
06
01
2020
accepted:
07
01
2020
entrez:
16
1
2020
pubmed:
16
1
2020
medline:
11
3
2021
Statut:
epublish
Résumé
The niche is a specialized microenvironment for tissue stem cells in vivo. It has long been emphasized that niche ECM molecules act on tissue stem cells to regulate their behavior, but the molecular entities of these interactions remain to be fully elucidated. Here, we report that laminin forms the in vivo ECM niche for trophoblast stem cells (TSCs), the tissue stem cells of the placenta. TSCs expressed fibronectin-binding, vitronectin-binding, and laminin-binding integrins, whereas the integrin ligands present in the TSC niche were collagen and laminin. Therefore, the only niche integrin ligand available for TSCs in vivo was laminin. Laminin promoted TSC adhesion and proliferation in vitro in an integrin binding-dependent manner. Importantly, when the integrin-binding ability of laminin was genetically ablated in mice, the size of the TSC population was significantly reduced compared with that in control mice. The present findings underscore an ECM niche function of laminin to support tissue stem cell maintenance in vivo.
Identifiants
pubmed: 31937556
pii: 3/2/e201900515
doi: 10.26508/lsa.201900515
pmc: PMC6977391
pii:
doi:
Substances chimiques
Integrins
0
Laminin
0
laminin gamma 1
0
Collagen
9007-34-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2020 Kiyozumi et al.
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