High-yield Production of Amyloid-β Peptide Enabled by a Customized Spider Silk Domain.

Amino Acid Sequence Amyloid beta-Peptides / metabolism Animals Fibroins / chemistry Models, Molecular Protein Domains

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
14 01 2020
Historique:
received: 12 10 2019
accepted: 18 12 2019
entrez: 16 1 2020
pubmed: 16 1 2020
medline: 11 11 2020
Statut: epublish

Résumé

During storage in the silk gland, the N-terminal domain (NT) of spider silk proteins (spidroins) keeps the aggregation-prone repetitive region in solution at extreme concentrations. We observe that NTs from different spidroins have co-evolved with their respective repeat region, and now use an NT that is distantly related to previously used NTs, for efficient recombinant production of the amyloid-β peptide (Aβ) implicated in Alzheimer's disease. A designed variant of NT from Nephila clavipes flagelliform spidroin, which in nature allows production and storage of β-hairpin repeat segments, gives exceptionally high yields of different human Aβ variants as a solubility tag. This tool enables efficient production of target peptides also in minimal medium and gives up to 10 times more isotope-labeled monomeric Aβ peptides per liter bacterial culture than previously reported.

Identifiants

DOI: 10.1038/s41598-019-57143-x PMID: 31937841 PMC: PMC6959368
pubmed: 31937841
doi: 10.1038/s41598-019-57143-x
pii: 10.1038/s41598-019-57143-x
pmc: PMC6959368
doi:

Substances chimiques

Amyloid beta-Peptides 0
Fibroins 9007-76-5

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

235

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Auteurs

Axel Abelein (A)

Department of Neurobiology, Care Sciences and Society, Center for Alzheimer Research, Division of Neurogeriatrics, Karolinska Institutet, 141 52, Huddinge, Sweden.

Gefei Chen (G)

Department of Neurobiology, Care Sciences and Society, Center for Alzheimer Research, Division of Neurogeriatrics, Karolinska Institutet, 141 52, Huddinge, Sweden.

Kristīne Kitoka (K)

Department of Physical Organic Chemistry, Latvian Institute of Organic Synthesis, Riga, LV-1006, Latvia.

Rihards Aleksis (R)

Department of Physical Organic Chemistry, Latvian Institute of Organic Synthesis, Riga, LV-1006, Latvia.

Filips Oleskovs (F)

Department of Physical Organic Chemistry, Latvian Institute of Organic Synthesis, Riga, LV-1006, Latvia.

Médoune Sarr (M)

Department of Neurobiology, Care Sciences and Society, Center for Alzheimer Research, Division of Neurogeriatrics, Karolinska Institutet, 141 52, Huddinge, Sweden.

Michael Landreh (M)

Department of Molecular Tumor and Cell Biology, Karolinska Institutet, 171 65, Solna, Sweden.

Jens Pahnke (J)

Department of Pathology, University of Oslo/Oslo University Hospital, N-0424, Oslo, Norway.
LIED, University of Lübeck, D-23538, Lübeck, Germany.
Department of Pharmacology, Medical Faculty, University of Latvia, Riga, LV-1004, Latvia.

Kerstin Nordling (K)

Department of Neurobiology, Care Sciences and Society, Center for Alzheimer Research, Division of Neurogeriatrics, Karolinska Institutet, 141 52, Huddinge, Sweden.

Nina Kronqvist (N)

Department of Neurobiology, Care Sciences and Society, Center for Alzheimer Research, Division of Neurogeriatrics, Karolinska Institutet, 141 52, Huddinge, Sweden.

Kristaps Jaudzems (K)

Department of Physical Organic Chemistry, Latvian Institute of Organic Synthesis, Riga, LV-1006, Latvia.

Anna Rising (A)

Department of Neurobiology, Care Sciences and Society, Center for Alzheimer Research, Division of Neurogeriatrics, Karolinska Institutet, 141 52, Huddinge, Sweden.
Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, 750 07, Uppsala, Sweden.

Jan Johansson (J)

Department of Neurobiology, Care Sciences and Society, Center for Alzheimer Research, Division of Neurogeriatrics, Karolinska Institutet, 141 52, Huddinge, Sweden.

Henrik Biverstål (H)

Department of Neurobiology, Care Sciences and Society, Center for Alzheimer Research, Division of Neurogeriatrics, Karolinska Institutet, 141 52, Huddinge, Sweden. henrik.biverstal@ki.se.
Department of Physical Organic Chemistry, Latvian Institute of Organic Synthesis, Riga, LV-1006, Latvia. henrik.biverstal@ki.se.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › High-yield Production of Amyloid-β Peptide...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Peptides bêta-amyloïdes › High-yield Production of Amyloid-β Peptide...
questionsmedicales.fr › Eucaryotes › Animaux › High-yield Production of Amyloid-β Peptide...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'arthropode › Soie › Fibroïne › High-yield Production of Amyloid-β Peptide...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › High-yield Production of Amyloid-β Peptide...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure tertiaire des protéines › Domaines protéiques › High-yield Production of Amyloid-β Peptide...