Localization of ATP-sensitive K
ATP-sensitive K+ channel
Hepatic stellate cells
Kupffer cells
Liver
Rat
Sinusoidal endothelial cells
Journal
World journal of experimental medicine
ISSN: 2220-315X
Titre abrégé: World J Exp Med
Pays: United States
ID NLM: 101618808
Informations de publication
Date de publication:
19 Dec 2019
19 Dec 2019
Historique:
received:
17
05
2019
revised:
05
09
2019
accepted:
20
11
2019
entrez:
16
1
2020
pubmed:
16
1
2020
medline:
16
1
2020
Statut:
epublish
Résumé
ATP-sensitive K To investigate the expression of K Rabbit anti-rat SUR1 peptide antibody was raised and purified by antigen immunoaffinity column chromatography. Four of Sprague-Dawley rats were used for liver protein extraction for immunoblot analysis, seven of them were used for immunohistochemistry both for the ABC method and immunofluorescence staining. Four of Wistar rats were used for the isolation of hepatic stellate cells (HSCs) and Kupffer cells for both primary culture and immunocytochemistry. Immunoblot analysis showed that the five kinds of K Observations from the present study indicated that K
Sections du résumé
BACKGROUND
BACKGROUND
ATP-sensitive K
AIM
OBJECTIVE
To investigate the expression of K
METHODS
METHODS
Rabbit anti-rat SUR1 peptide antibody was raised and purified by antigen immunoaffinity column chromatography. Four of Sprague-Dawley rats were used for liver protein extraction for immunoblot analysis, seven of them were used for immunohistochemistry both for the ABC method and immunofluorescence staining. Four of Wistar rats were used for the isolation of hepatic stellate cells (HSCs) and Kupffer cells for both primary culture and immunocytochemistry.
RESULTS
RESULTS
Immunoblot analysis showed that the five kinds of K
CONCLUSION
CONCLUSIONS
Observations from the present study indicated that K
Identifiants
pubmed: 31938690
doi: 10.5493/wjem.v9.i2.14
pmc: PMC6955576
doi:
Types de publication
Journal Article
Langues
eng
Pagination
14-31Informations de copyright
©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict-of-interest statement: Authors declare no conflicts of interest for this article.
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