Adaption of an autonomously cascade DNA circuit for amplified detection and intracellular imaging of polynucleotide kinase with ultralow background.
Amplification
Hybridization chain reaction
Intracellular imaging
Polynucleotide kinase
Sensor
Journal
Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289
Informations de publication
Date de publication:
15 Mar 2020
15 Mar 2020
Historique:
received:
07
11
2019
revised:
15
12
2019
accepted:
23
12
2019
pubmed:
17
1
2020
medline:
15
12
2020
entrez:
17
1
2020
Statut:
ppublish
Résumé
Polynucleotide kinase (PNK) plays a crucial role in phosphorylation-related DNA repair, while its real time tracking and monitoring is unexplored for limited sensitivity and robustness of current PNK sensing strategies in complex biological environment. Herein, we proposed a concatenated hybridization chain reaction (Con-HCR)-based PNK sensing platform for ultra-sensitive PNK assay and intracellular imaging. In the presence of PNK, the 5'-hydroxyl termini of hairpin H
Identifiants
pubmed: 31941614
pii: S0956-5663(19)31071-1
doi: 10.1016/j.bios.2019.111994
pii:
doi:
Substances chimiques
DNA
9007-49-2
Polynucleotide 5'-Hydroxyl-Kinase
EC 2.7.1.78
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111994Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.