Regenerative and protective effects of calcium silicate on senescent fibroblasts induced by high glucose.
Animals
Calcium Compounds
/ therapeutic use
Cell Culture Techniques
Cell Differentiation
/ drug effects
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Cellular Senescence
Diabetes Complications
/ complications
Disease Models, Animal
Female
Fibroblasts
/ drug effects
Glucose
/ pharmacology
Humans
Male
Mice
Myofibroblasts
/ drug effects
Silicates
/ therapeutic use
Smad2 Protein
Surgical Wound
/ etiology
Wound Healing
/ drug effects
Journal
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
ISSN: 1524-475X
Titre abrégé: Wound Repair Regen
Pays: United States
ID NLM: 9310939
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
03
06
2019
revised:
13
12
2019
accepted:
29
12
2019
pubmed:
17
1
2020
medline:
7
8
2021
entrez:
17
1
2020
Statut:
ppublish
Résumé
Diabetic wounds are a common complication of diabetes and therefore a pressing issue for clinicians. High-glucose (HG)-induced fibroblast senescence is mainly responsible for delayed wound healing. Calcium silicate (CS), a kind of bioceramic, is thought to have regenerative properties. The aim of this study was to determine the regenerative and protective effects of CS on senescent fibroblasts induced by HG. Fibroblasts were passaged five times and treated with HG and CS. Compared with the normal glucose (NG) group, the proliferation, migration, and differentiation capacity of HG-induced fibroblasts significantly decreased (P < .05). After treatment with CS, the functions of HG-induced senescent fibroblasts were partly restored (P < .05). The mechanism of the regenerative and protective effects of CS may be related to the decreased reactive oxygen species generation, improved senescent state (SA-β-gal expression decreased), up-regulated expression of Smad2 and phosphorylated Smad2, and down-regulated expression of p16, p21, and p53. An in vivo experiment also demonstrated that CS had a therapeutic effect on diabetic wounds via differentiation of fibroblasts into myofibroblasts and enhanced collagen deposition. These results indicate that CS may be a promising candidate for diabetic wound therapy.
Substances chimiques
Calcium Compounds
0
SMAD2 protein, human
0
Silicates
0
Smad2 Protein
0
Glucose
IY9XDZ35W2
calcium silicate
S4255P4G5M
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
315-325Informations de copyright
© 2020 by the Wound Healing Society.
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