In vitro assays for effector T cell functions and activity of immunomodulatory antibodies.
Co-stimulation
GITR
Immune suppression
Immunotherapy
T cells
Tregs
Journal
Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
18
1
2020
pubmed:
18
1
2020
medline:
5
1
2021
Statut:
ppublish
Résumé
The recent clinical success of cancer immunotherapy with checkpoint blockade has led to renewed interest into the development of immune modulatory agents with the capacity to activate anti-tumor T cell responses. Standardization of optimized in vitro assays for efficient assessment of immune function of such new drugs is thus needed to facilitate clinical development of the optimal drug candidates. Here, we describe an optimized version of T cell suppression assay designed to test the effect of immunomodulatory agents on T cell function and activation. We apply this assay to investigate the agonist activity of the T cell co-stimulatory molecule glucocorticoid-induced TNFR-related protein (GITR). We detail a protocol for concurrent assessment of multiple levels of T cell functional modulation upon GITR engagement, including T cell priming, activation and effector function, in a single assay. As human GITR agonist antibodies are currently under development, availability of standardized cell-based functional assays of GITR agonism is instrumental to translate anti-GITR therapy into the clinical setting.
Identifiants
pubmed: 31948562
pii: S0076-6879(19)30357-X
doi: 10.1016/bs.mie.2019.08.012
pmc: PMC7362725
mid: NIHMS1558165
pii:
doi:
Substances chimiques
Antibodies, Monoclonal
0
Glucocorticoid-Induced TNFR-Related Protein
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
43-59Subventions
Organisme : NCI NIH HHS
ID : R25 CA020449
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA215136
Pays : United States
Organisme : NCI NIH HHS
ID : P01 CA033049
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NCI NIH HHS
ID : P01 CA059350
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA056821
Pays : United States
Informations de copyright
© 2020 Elsevier Inc. All rights reserved.
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