Evaluation of the U.S. Food and Drug Administration validated molecular method for detection of Cyclospora cayetanensis oocysts on fresh and frozen berries.
Cyclospora cayetanensis
Fresh berries
Frozen berries
Molecular detection
Journal
Food microbiology
ISSN: 1095-9998
Titre abrégé: Food Microbiol
Pays: England
ID NLM: 8601127
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
21
08
2019
revised:
12
11
2019
accepted:
01
12
2019
entrez:
18
1
2020
pubmed:
18
1
2020
medline:
27
2
2020
Statut:
ppublish
Résumé
Outbreaks and sporadic cases of Cyclospora cayetanensis have been linked to consumption of berries. The efficacy of the U.S. Food and Drug Administration (FDA) method for detection of C. cayetanensis was evaluated in fresh berries (blackberries, strawberries, blueberries and mixed berries) and in frozen mixed berries. The protocol included seeding with C. cayetanensis oocysts, produce washing, DNA extraction and a dual TaqMan assay. As few as five oocysts were detected in every type of fresh berry analyzed. All berry samples seeded with 200 oocysts were positive and all unseeded berry samples were negative. No significant differences were observed among any of the berry types analyzed in detection rates, C
Identifiants
pubmed: 31948637
pii: S0740-0020(19)31007-X
doi: 10.1016/j.fm.2019.103397
pii:
doi:
Types de publication
Journal Article
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
103397Informations de copyright
Published by Elsevier Ltd.