Tunable light and drug induced depletion of target proteins.
Animals
Apoptosis
Caenorhabditis elegans
/ genetics
Caenorhabditis elegans Proteins
/ drug effects
Caspases
/ drug effects
Cell Engineering
/ methods
Cytological Techniques
DNA Damage
DNA Ligase ATP
DNA Repair
DNA-Binding Proteins
/ metabolism
Gene Expression Regulation, Developmental
Green Fluorescent Proteins
HeLa Cells
Humans
Lamin Type A
/ metabolism
Light
Proliferating Cell Nuclear Antigen
/ metabolism
Transcription Factors
/ metabolism
Ubiquitin-Protein Ligases
/ drug effects
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
16 01 2020
16 01 2020
Historique:
received:
06
05
2019
accepted:
12
12
2019
entrez:
18
1
2020
pubmed:
18
1
2020
medline:
9
4
2020
Statut:
epublish
Résumé
Biological processes in development and disease are controlled by the abundance, localization and modification of cellular proteins. We have developed versatile tools based on recombinant E3 ubiquitin ligases that are controlled by light or drug induced heterodimerization for nanobody or DARPin targeted depletion of endogenous proteins in cells and organisms. We use this rapid, tunable and reversible protein depletion for functional studies of essential proteins like PCNA in DNA repair and to investigate the role of CED-3 in apoptosis during Caenorhabditis elegans development. These independent tools can be combined for spatial and temporal depletion of different sets of proteins, can help to distinguish immediate cellular responses from long-term adaptation effects and can facilitate the exploration of complex networks.
Identifiants
pubmed: 31949141
doi: 10.1038/s41467-019-14160-8
pii: 10.1038/s41467-019-14160-8
pmc: PMC6965615
doi:
Substances chimiques
CXXC4 protein, human
0
Caenorhabditis elegans Proteins
0
DNA-Binding Proteins
0
LIG1 protein, human
0
Lamin Type A
0
PCN-1 protein, C elegans
0
Proliferating Cell Nuclear Antigen
0
Transcription Factors
0
Green Fluorescent Proteins
147336-22-9
Ubiquitin-Protein Ligases
EC 2.3.2.27
Caspases
EC 3.4.22.-
ced-3 protein, C elegans
EC 3.4.22.-
DNA Ligase ATP
EC 6.5.1.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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