The Activity of Hydrolyzed Virgin Coconut Oil to Increase Proliferation and Cyclooxygenase-2 Expression towards on NIH 3T3 Cell Line in Wound Healing Process.
COX-2
Cell proliferation
HVCO
NIH 3T3
Percent of the wound closed
Journal
Open access Macedonian journal of medical sciences
ISSN: 1857-9655
Titre abrégé: Open Access Maced J Med Sci
Pays: North Macedonia
ID NLM: 101662294
Informations de publication
Date de publication:
15 Oct 2019
15 Oct 2019
Historique:
received:
13
06
2019
revised:
20
07
2019
accepted:
21
07
2019
entrez:
18
1
2020
pubmed:
18
1
2020
medline:
18
1
2020
Statut:
epublish
Résumé
This study aims to determine the effect of hydrolysed virgin coconut oil (HVCO) to increase cell proliferation, COX-2 expression of NIH 3T3. The sample used was Virgin Coconut Oil (VCO). VCO was partially hydrolysed using lipase from Acid value is 135.89 ± 0.12 mg NaOH/g oil and free fatty acids (FFA) is 48.50 ± 0.06%. The effect of HVCO 62.5 µg/mL on cell proliferation after 24h, 48h, and 72h incubation found as viable cells are 109.24 ± 0.52%; 118.26 ± 0.91% and 106.59 ± 0.74%. Percent of wound closed after 24 h and 48 h incubation are 69.94 ± 0.54% and 100.00 ± 0.00%, and expression of COX-2 increased from 1 (control) to 1.83 (HVCO). The results suggest that HVCO is effective to increase cells proliferation and hence wound healing process.
Identifiants
pubmed: 31949510
doi: 10.3889/oamjms.2019.804
pii: OAMJMS-7-3164
pmc: PMC6953946
doi:
Types de publication
Journal Article
Langues
eng
Pagination
3164-3168Informations de copyright
Copyright: © 2019 Jansen Silalahi, Yuandani Yuandani, Dian Ika Perbina Br Meliala, Linda Margata, Denny Satria.
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