Effect of Single Injection of Recombinant Human Bone Morphogenetic Protein-2-Loaded Artificial Collagen-Like Peptide in a Mouse Segmental Bone Transport Model.
Animals
Bone Density
/ drug effects
Bone Morphogenetic Protein 2
/ chemistry
Bone Regeneration
/ drug effects
Collagen
/ chemistry
Disease Models, Animal
Femur
/ drug effects
Fracture Healing
/ drug effects
Humans
Mice
Peptides
/ chemistry
Recombinant Proteins
/ chemistry
Skull
/ drug effects
Transforming Growth Factor beta
/ chemistry
X-Ray Microtomography
Journal
BioMed research international
ISSN: 2314-6141
Titre abrégé: Biomed Res Int
Pays: United States
ID NLM: 101600173
Informations de publication
Date de publication:
2019
2019
Historique:
received:
21
08
2019
accepted:
09
12
2019
entrez:
18
1
2020
pubmed:
18
1
2020
medline:
3
7
2020
Statut:
epublish
Résumé
This study aimed to investigate whether a single injection of recombinant human bone morphogenetic protein-2-loaded artificial collagen-like peptide gel (rhBMP-2/ACG) accelerates consolidation at the bone defect site and bone union at the docking site in a mouse segmental bone transport (SBT) model. A critical sized bone defect (2 mm) was created in the femur of mice and subsequently reconstructed using SBT with an external fixator. Mice were divided into four treatment groups: Group CONT (immobile control), Group 0.2 (bone segments moved 0.2 mm/day for 10 days), Group 1.0 (bone segments moved 1.0 mm/day for 2 days), and Group 1.0/BMP-2 (rhBMP-2/ACG injected into the bone defect and segments moved 1.0 mm/day for 2 days). Consolidation at the bone defect site and bone union at the docking site was evaluated radiologically and histologically across eight weeks. Bone volume and bone mineral content were significantly higher in Group 0.2 than in Group 1.0. Group 0.2 showed evidence of rebuilding of the medullary canal eight weeks after surgery at the bone defect site. However, in Group 1.0, maturation of regenerative bone at the bone defect site was poor, with the central area between the proximal and distal bone composed mainly of masses of fibrous and adipose tissue. Group 1.0/BMP-2 had higher bone volume and bone mineral content compared to Group 1.0, and all mice achieved bone union at the bone defect and docking sites. Single injection of rhBMP-2/ACG combined with SBT may be effective for enhancing bone healing in large bone defects.
Identifiants
pubmed: 31950029
doi: 10.1155/2019/1014594
pmc: PMC6948306
doi:
Substances chimiques
Bone Morphogenetic Protein 2
0
Peptides
0
Recombinant Proteins
0
Transforming Growth Factor beta
0
recombinant human bone morphogenetic protein-2
0
Collagen
9007-34-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1014594Informations de copyright
Copyright © 2019 Ryo Tazawa et al.
Déclaration de conflit d'intérêts
The authors declare that they have no conflicts of interest.
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