Fabrication of Gentamicin-Loaded Hydroxyapatite/Collagen Bone-Like Nanocomposite for Anti-Infection Bone Void Fillers.
Adsorption
Anti-Bacterial Agents
/ chemistry
Bone Substitutes
/ chemistry
Buffers
Cell Line, Tumor
Cell Survival
/ drug effects
Collagen
/ chemistry
Drug Liberation
Durapatite
/ chemistry
Escherichia coli
/ drug effects
Gentamicins
/ chemistry
Humans
Infections
/ drug therapy
Nanocomposites
/ chemistry
Phosphates
/ chemistry
Powders
antibacterial activity
antibiotic-impregnated biomaterial
bone void filler
gentamicin sulfate
hydroxyapatite/collagen bone-like nanocomposite
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
15 Jan 2020
15 Jan 2020
Historique:
received:
24
09
2019
revised:
09
01
2020
accepted:
09
01
2020
entrez:
19
1
2020
pubmed:
19
1
2020
medline:
6
10
2020
Statut:
epublish
Résumé
A gentamicin-loaded hydroxyapatite/collagen bone-like nanocomposite (GNT-HAp/Col) was fabricated and evaluated for its absorption-desorption properties, antibacterial efficacy, and cytotoxicity. The hydroxyapatite/collagen bone-like nanocomposite (HAp/Col) powder was mixed with gentamicin sulfate (GNT) in phosphate-buffered saline (PBS) at room temperature. After 6 h mixing, the GNT adsorption in all conditions reached plateau by Langmuir's isotherm, and maximum GNT adsorption amount was 34 ± 7 μg in 250 μg/mL GNT solution. Saturated GNT-loaded HAp/Col powder of 100 mg was soaked in 10 mL of PBS at 37 °C and released all GNT in 3 days. A shaking culture method for a GNT extraction from the GNT-HAp/Col and an inhibition zone assay for the GNT-HAp/Col compact showed antibacterial efficacy to
Identifiants
pubmed: 31952242
pii: ijms21020551
doi: 10.3390/ijms21020551
pmc: PMC7013509
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bone Substitutes
0
Buffers
0
Gentamicins
0
Phosphates
0
Powders
0
Collagen
9007-34-5
Durapatite
91D9GV0Z28
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
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