Visualization of Leishmania tropica Infection in BALB/c Mice by Bioluminescence Imaging
Animals
Diagnostic Imaging
Disease Models, Animal
Female
Green Fluorescent Proteins
/ metabolism
Leishmania tropica
/ pathogenicity
Leishmaniasis, Cutaneous
/ diagnostic imaging
Luciferases
/ metabolism
Luminescent Measurements
Lymph Nodes
/ parasitology
Mice, Inbred BALB C
Parasites
/ pathogenicity
Journal
Iranian biomedical journal
ISSN: 2008-823X
Titre abrégé: Iran Biomed J
Pays: Iran
ID NLM: 9814853
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
entrez:
19
1
2020
pubmed:
19
1
2020
medline:
18
12
2020
Statut:
ppublish
Résumé
Leishmania tropica is the cause of more than one form of leishmaniasis and lacks a known reservoir animal. This study compares the potential infectivity of recombinant and wild-type L. tropica in BALB/c
mice. The potential infectivity of recombinant L. tropicaEGFP or L. tropicaEGFP-LUC by two different, the subcutaneous and intradermal, routes was compared using a range of classical detection methods and bioluminescence imaging (BLI). In addition to the results obtained from classical diagnostic approaches, the BLI signals were detected in footpads and ears of L. tropica-infected animals. The BLI revealed that a bioluminescence signal can be observed at the inoculation site. The stability of the BLI remained constant in the footpad, but the signal was detectable for only three months in the pinna due to the decline in infection over time. The presented data are a precise verification of the assumption that BALB/c mice could be used as an experimental model for L. tropica infectivity.
Sections du résumé
Background
Leishmania tropica is the cause of more than one form of leishmaniasis and lacks a known reservoir animal. This study compares the potential infectivity of recombinant and wild-type L. tropica in BALB/c
mice.
Methods
The potential infectivity of recombinant L. tropicaEGFP or L. tropicaEGFP-LUC by two different, the subcutaneous and intradermal, routes was compared using a range of classical detection methods and bioluminescence imaging (BLI).
Results
In addition to the results obtained from classical diagnostic approaches, the BLI signals were detected in footpads and ears of L. tropica-infected animals. The BLI revealed that a bioluminescence signal can be observed at the inoculation site. The stability of the BLI remained constant in the footpad, but the signal was detectable for only three months in the pinna due to the decline in infection over time.
Conclusion
The presented data are a precise verification of the assumption that BALB/c mice could be used as an experimental model for L. tropica infectivity.
Substances chimiques
enhanced green fluorescent protein
0
Green Fluorescent Proteins
147336-22-9
Luciferases
EC 1.13.12.-
Types de publication
Journal Article
Langues
eng
Pagination
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