Virulent coliphages in 1-year-old children fecal samples are fewer, but more infectious than temperate coliphages.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
17 01 2020
17 01 2020
Historique:
received:
12
06
2019
accepted:
10
12
2019
entrez:
19
1
2020
pubmed:
19
1
2020
medline:
10
4
2020
Statut:
epublish
Résumé
Bacteriophages constitute an important part of the human gut microbiota, but their impact on this community is largely unknown. Here, we cultivate temperate phages produced by 900 E. coli strains isolated from 648 fecal samples from 1-year-old children and obtain coliphages directly from the viral fraction of the same fecal samples. We find that 63% of strains hosted phages, while 24% of the viromes contain phages targeting E. coli. 150 of these phages, half recovered from strain supernatants, half from virome (73% temperate and 27% virulent) were tested for their host range on 75 E. coli strains isolated from the same cohort. Temperate phages barely infected the gut strains, whereas virulent phages killed up to 68% of them. We conclude that in fecal samples from children, temperate coliphages dominate, while virulent ones have greater infectivity and broader host range, likely playing a role in gut microbiota dynamics.
Identifiants
pubmed: 31953385
doi: 10.1038/s41467-019-14042-z
pii: 10.1038/s41467-019-14042-z
pmc: PMC6969025
doi:
Substances chimiques
Carrier Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
378Références
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