Bacterial Inclusion Bodies: A Treasure Trove of Bioactive Proteins.
bacterial inclusion bodies
combinatorial approach
mild solubilization agents
protein aggregates
protein purification
protein refolding
Journal
Trends in biotechnology
ISSN: 1879-3096
Titre abrégé: Trends Biotechnol
Pays: England
ID NLM: 8310903
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
18
09
2019
revised:
29
11
2019
accepted:
06
12
2019
pubmed:
20
1
2020
medline:
31
7
2021
entrez:
20
1
2020
Statut:
ppublish
Résumé
Recombinant proteins expressed as bacterial inclusion bodies (IBs) are now receiving tremendous attention for many diverse applications in the areas of industrial and medical biotechnology. Understanding the structure-function relationship of protein in IBs has recently created new possibilities in developing innovative isolation, solubilization, refolding, and purification processes for high-throughput recovery of bioactive protein from bacterial IBs. This opinion article describes the advantages, disadvantages, and major challenges presently associated with each of the processing steps. Finally, we conclude with the possible solutions for each operational step and the future direction of the basic and translational research to achieve maximum benefit from IB aggregates.
Identifiants
pubmed: 31954528
pii: S0167-7799(19)30306-3
doi: 10.1016/j.tibtech.2019.12.011
pii:
doi:
Substances chimiques
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
474-486Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.