Cryo-EM structures of cardiac thin filaments reveal the 3D architecture of troponin.
Actin
Muscle
Single particle analysis
Tropomyosin
Volta phase plate
Journal
Journal of structural biology
ISSN: 1095-8657
Titre abrégé: J Struct Biol
Pays: United States
ID NLM: 9011206
Informations de publication
Date de publication:
01 03 2020
01 03 2020
Historique:
received:
05
12
2019
revised:
06
01
2020
accepted:
12
01
2020
pubmed:
20
1
2020
medline:
17
4
2021
entrez:
20
1
2020
Statut:
ppublish
Résumé
Troponin is an essential component of striated muscle and it regulates the sliding of actomyosin system in a calcium-dependent manner. Despite its importance, the structure of troponin has been elusive due to its high structural heterogeneity. In this study, we analyzed the 3D structures of murine cardiac thin filaments using a cryo-electron microscope equipped with a Volta phase plate (VPP). Contrast enhancement by a VPP enabled us to reconstruct the entire repeat of the thin filament. We determined the orientation of troponin relative to F-actin and tropomyosin, and characterized the interactions between troponin and tropomyosin. This study provides a structural basis for understanding the molecular mechanism of actomyosin system.
Identifiants
pubmed: 31954841
pii: S1047-8477(20)30004-6
doi: 10.1016/j.jsb.2020.107450
pii:
doi:
Substances chimiques
Actins
0
Tropomyosin
0
Troponin
0
Actomyosin
9013-26-7
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107450Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.