NADPH biosensor-based identification of an alcohol dehydrogenase variant with improved catalytic properties caused by a single charge reversal at the protein surface.
Enzyme optimization
Fluorescence-activated cell sorting
Lactobacillus brevis
NADPH biosensor
NADPH-dependent alcohol dehydrogenase
Random mutagenesis
Journal
AMB Express
ISSN: 2191-0855
Titre abrégé: AMB Express
Pays: Germany
ID NLM: 101561785
Informations de publication
Date de publication:
18 Jan 2020
18 Jan 2020
Historique:
received:
20
08
2019
accepted:
06
01
2020
entrez:
20
1
2020
pubmed:
20
1
2020
medline:
20
1
2020
Statut:
epublish
Résumé
Alcohol dehydrogenases (ADHs) are used in reductive biotransformations for the production of valuable chiral alcohols. In this study, we used a high-throughput screening approach based on the NADPH biosensor pSenSox and fluorescence-activated cell sorting (FACS) to search for variants of the NADPH-dependent ADH of Lactobacillus brevis (LbADH) with improved activity for the reduction of 2,5-hexanedione to (2R,5R)-hexanediol. In a library of approx. 1.4 × 10
Identifiants
pubmed: 31955268
doi: 10.1186/s13568-020-0946-7
pii: 10.1186/s13568-020-0946-7
pmc: PMC6969876
doi:
Types de publication
Journal Article
Langues
eng
Pagination
14Subventions
Organisme : Bundesministerium für Bildung und Forschung
ID : 031A095B
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