Protective effect of Agrimonia pilosa polysaccharides on dexamethasone-treated MC3T3-E1 cells via Wnt/β-Catenin pathway.
Agrimonia pilosa polysaccharide
Wnt/β-Catenin signalling pathway
apoptosis
steroid-induced avascular necrosis of the femoral head (SANFH)
sulphated polysaccharide
Journal
Journal of cellular and molecular medicine
ISSN: 1582-4934
Titre abrégé: J Cell Mol Med
Pays: England
ID NLM: 101083777
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
08
10
2019
revised:
09
11
2019
accepted:
11
11
2019
pubmed:
21
1
2020
medline:
28
4
2021
entrez:
21
1
2020
Statut:
ppublish
Résumé
A water-soluble polysaccharide (APP-AW) was isolated from Agrimonia pilosa and prepared to three sulphated derivatives (S1, S2 and S3). The results showed that pre-treatment with APP-AW, S1, S2 and S3 each at the concentration of 50 μg/mL for 48 hours was able to prevent cytotoxicity induced by 1 μmol/L dexamethasone (Dex) in MC3T3-E1 cells via inhibition of apoptosis, which is in line with the findings in flow cytometry analysis. Meanwhile, the decreased ALP activity, collagen content, mineralization, BMP2, Runx2, OSX and OCN protein expression in DEX-treated MC3T3-E1 cells were reversed by the addition of APP-AW, S1, S2 and S3. Moreover, APP-AW, S1, S2 and S3 rescued DEX-induced increase of Bax, cytochrome c and caspase-3 and decrease of Bcl-2, Wnt3, β-catenin and c-Myc protein expression in MC3T3-E1 cells. Our findings suggest that pre-treatment with APP-AW, S1, S2 and S3 could significantly protect MC3T3-E1 cells against Dex-induced cell injury via inhibiting apoptosis and activating Wnt/β-Catenin signalling pathway, thus application of these polysaccharides may be a promising alternative strategy for steroid-induced avascular necrosis of the femoral head (SANFH) therapy.
Identifiants
pubmed: 31957180
doi: 10.1111/jcmm.14868
pmc: PMC7011133
doi:
Substances chimiques
CTNNB1 protein, mouse
0
Polysaccharides
0
Protective Agents
0
beta Catenin
0
Dexamethasone
7S5I7G3JQL
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2169-2177Informations de copyright
© 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
Références
Tumour Biol. 2016 Apr;37(4):5239-45
pubmed: 26555544
Expert Opin Ther Targets. 2009 Apr;13(4):485-96
pubmed: 19335070
Int J Biol Macromol. 2018 Feb;107(Pt A):713-718
pubmed: 28923567
J Cell Mol Med. 2020 Feb;24(3):2169-2177
pubmed: 31957180
ScientificWorldJournal. 2014;2014:347813
pubmed: 25110730
Carbohydr Polym. 2019 Jun 15;214:71-79
pubmed: 30926009
Lancet. 1984 May 19;1(8386):1091-3
pubmed: 6144827
FASEB J. 1990 Oct;4(13):3111-23
pubmed: 2210157
Lancet Diabetes Endocrinol. 2013 Sep;1(1):59-70
pubmed: 24622268
Mol Med Rep. 2015 Dec;12(6):8155-61
pubmed: 26496816
Exp Ther Med. 2017 Jan;13(1):69-74
pubmed: 28123470
Exp Mol Pathol. 2014 Feb;96(1):9-14
pubmed: 24200492
Proc Natl Acad Sci U S A. 1990 Jul;87(13):5129-33
pubmed: 2367528
Cell Physiol Biochem. 2017;43(4):1547-1561
pubmed: 29035884
Int J Biol Macromol. 2009 Jan 1;44(1):14-7
pubmed: 18951914
J Orthop Sci. 2007 May;12(3):289-95
pubmed: 17530382
Int J Biol Macromol. 2019 Feb 15;123:581-586
pubmed: 30419325
J Clin Invest. 2006 May;116(5):1202-9
pubmed: 16670761
Int J Biol Macromol. 2018 Sep;116:106-112
pubmed: 29729342
Int J Mol Med. 2012 Mar;29(3):435-9
pubmed: 22109711
J Bone Joint Surg Am. 2008 Nov;90 Suppl 4:175-87
pubmed: 18984729
J Enzyme Inhib Med Chem. 2017 Dec;32(1):707-711
pubmed: 28385082
Int J Mol Med. 2012 Oct;30(4):889-95
pubmed: 22842877
Anal Biochem. 1969 Nov;32(2):314-21
pubmed: 5396941
Am J Med. 1986 Mar;80(3):511-3
pubmed: 3953626